PXD032372

PXD032372 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of tau and Aβ amyloid in detergent insoluble fractions from Alzheimer’s disease brains
Description	The two hallmarks of Alzheimer’s disease (AD) are A-amyloid- (A) plaques and neurofibrillary tangles marked by phosphorylated tau. There is compelling evidence that aggregating A drives tau accumulation, a process that involves synaptic degeneration leading to cognitive impairment. Conversely there is a growing realization that non-fibrillar (oligomeric) forms of Aβ mediate toxicity in AD. Using quantitative proteomics, we find that AD brain sarkosyl-insoluble pellets are greatly enriched with A at almost equimolar levels to N-terminal truncated microtubule binding region (MTBR) isoforms of tau with multiple post-translational modifications (PTMs). The extracted R3-R4 tau peptides are 100-fold higher compared to N-terminus intact tau peptides. Substantial proportions of site-specific phosphorylation at T181 (~ 22 %) and T217 (~ 16 %) along with other PTMs in the proline rich region (PRR) and MTBR indicate a regional susceptibility of PTMs in aggregated tau. Immuno-electron microscopy reveals that co-purified A and tau co-localize to globular non-filamentous aggregates.
HostingRepository	PRIDE
AnnounceDate	2022-06-04
AnnouncementXML	Submission_2022-06-04_01:10:05.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soumya Mukherjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-17 09:40:35	ID requested
⏵ 1	2022-06-04 01:10:06	announced

Dataset with its publication pending

submitter keyword: tau, abeta, Quantitative proteomics

Colin Masters
contact affiliation	Neuropathology and Neurodegeneration Laboratory
contact email	c.masters@unimelb.edu.au
lab head
Soumya Mukherjee
contact affiliation	University of Melbourne
contact email	soumya.mukherjee1@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032372
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of tau and Aβ amyloid in detergent insoluble fractions from Alzheimer’s disease brains