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PXD032249

PXD032249 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOctreotide-induced changes in the saliva proteome
DescriptionSaliva was collected from n=3 subjects, before and after treatment with octreotide. 100 micrograms of saliva protein was reduced and alkylated, and digested with trypsin using an S-trap. Peptides (~45 ugs) were separated by microflow LC using a 1x100 mm Waters ACQUITY Premier CSH column at 100 uL/min using a gradient of 3-258 MeCN over 60 min. 3% DMSO was delivered post-column. The LC was interfaced to a Thermo Exploris 480 using a heated ESI source. MS/MS analysis used a staggered, overlapping DIA method with 60 K MS1 and 15K MS2 spanning 400-1000 m/z with 77 x 16 m/z windows and a 36 window cycle. Data was processed using Spectronaut Pulsar 15.
HostingRepositoryMassIVE
AnnounceDate2022-07-19
AnnouncementXMLSubmission_2022-07-19_07:42:48.242.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatt Foster
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Streptococcus mitis; NCBI TaxID: 28037; scientific name: Gamella haemolysans; scientific name: Granulicatella adiacens; NCBI TaxID: 46124;
ModificationListAcetyl; Carbamidomethyl; Oxidation
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-03-13 21:33:03ID requested
12022-07-19 07:42:48announced
Publication List
no publication
Keyword List
submitter keyword: microflow, data-independent acquisition
Contact List
Walter T. Lee
contact affiliationDuke University
contact emailwalter.lee@duke.edu
lab head
Matt Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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