PXD032249
PXD032249 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Octreotide-induced changes in the saliva proteome |
Description | Saliva was collected from n=3 subjects, before and after treatment with octreotide. 100 micrograms of saliva protein was reduced and alkylated, and digested with trypsin using an S-trap. Peptides (~45 ugs) were separated by microflow LC using a 1x100 mm Waters ACQUITY Premier CSH column at 100 uL/min using a gradient of 3-258 MeCN over 60 min. 3% DMSO was delivered post-column. The LC was interfaced to a Thermo Exploris 480 using a heated ESI source. MS/MS analysis used a staggered, overlapping DIA method with 60 K MS1 and 15K MS2 spanning 400-1000 m/z with 77 x 16 m/z windows and a 36 window cycle. Data was processed using Spectronaut Pulsar 15. |
HostingRepository | MassIVE |
AnnounceDate | 2022-07-19 |
AnnouncementXML | Submission_2022-07-19_07:42:48.242.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matt Foster |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Streptococcus mitis; NCBI TaxID: 28037; scientific name: Gamella haemolysans; scientific name: Granulicatella adiacens; NCBI TaxID: 46124; |
ModificationList | Acetyl; Carbamidomethyl; Oxidation |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-03-13 21:33:03 | ID requested | |
⏵ 1 | 2022-07-19 07:42:48 | announced |
Publication List
no publication |
Keyword List
submitter keyword: microflow, data-independent acquisition |
Contact List
Walter T. Lee | |
---|---|
contact affiliation | Duke University |
contact email | walter.lee@duke.edu |
lab head | |
Matt Foster | |
contact affiliation | Duke University |
contact email | mwfoster@duke.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089053/ |