PXD032236

PXD032236 is an original dataset announced via ProteomeXchange.

Title	Memory CD8+ T cells upregulate glycolysis and effector functions under limiting oxygen conditions
Description	Memory CD8+ T cells are indispensable for maintaining long-term immunity against intracellular pathogens and tumors. Despite their presence in oxygen-deprived tissues of infection sites or tumors, the impact of local oxygen pressure on memory CD8+ T cells has remained largely unclear. We sought to elucidate how oxygen pressure impacted memory CD8+ T cells arising after infection with Listeria monocytogenes-OVA. Our data revealed that reduced oxygen pressure during in vitro culture switched CD8+ T cell metabolism from an OXPHOS to a glycolytic phenotype. Quantitative proteomic analysis showed that limiting oxygen conditions increased the expression of glucose transporters and components of the glycolytic pathway, while decreasing TCA cycle and mitochondrial respiratory chain proteins. The altered CD8+ T cell metabolism did not affect the expansion potential, but enhanced the granzyme B and IFN- production capacity. Memory CD8+ T cells cultured under low oxygen pressure were able to persist long-term in vivo and provided protection against bacterial rechallenge. Taken together, our study indicates that strategies of cellular immune therapy may benefit from reducing oxygen during culture to develop memory CD8+ T cells with superior effector functions .
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:38.537.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Floris van Alphen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-03-11 10:56:52	ID requested
1	2022-12-01 03:54:16	announced
⏵ 2	2023-11-14 08:55:39	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Cytotoxic T cells,CD8+ memory T cells, Physioxic cell culture, TCR-mediated activation, T cell metabolism

Dr. Maartje van den Biggelaar
contact affiliation	Department of Molecular Hematology, Sanquin Research, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, the Netherlands
contact email	M.vandenbiggelaar@sanquin.nl
lab head
Floris van Alphen
contact affiliation	Sanquin Research
contact email	F.vanalphen@sanquin.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD032236
     1. Label: PRIDE project
     2. Name: Memory CD8+ T cells upregulate glycolysis and effector functions under limiting oxygen conditions