PXD031866

PXD031866 is an original dataset announced via ProteomeXchange.

Title	Stomatin modulates adipogenesis through ERK pathway and regulates fatty acid uptake and lipid droplet growth
Description	Controlling fatty acid uptake, lipid production and storage, and metabolism of lipid droplets (LDs), is closely related to lipid homeostasis, adipocyte hypertrophy and obesity. We report here that stomatin, a major constituent of lipid rafts, participates in adipogenesis and adipocyte maturation by modulating related signaling pathways. In adipocyte-like cells, increased stomatin promotes LD growth or enlargements by facilitating LD-LD fusion, as well as fatty acid uptake from extracellular environment by recruiting effector molecules, such as FAT/CD36 translocase, to lipid rafts to promote internalization of fatty acids. Stomatin transgenic mice fed with high-fat diets exhibit obesity, insulin resistance and hepatic impairments; however, such phenotypes are not seen if feeding the transgenic animals with regular diets. Inhibitions of stomatin by gene knockdown or OB-1 pharmacological treatments inhibit adipogenic differentiation and LD growth through downregulation of PPARγ pathway. Effects of stomatin on PPARγ involve ERK signaling; however, an alternate pathway may also exist.
HostingRepository	PRIDE
AnnounceDate	2022-07-05
AnnouncementXML	Submission_2022-07-05_08:10:03.041.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031866
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chen-Chung Liao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-24 01:05:37	ID requested
⏵ 1	2022-07-05 08:10:03	announced

Dataset with its publication pending

submitter keyword: Adipogenesis, ERK pathway, 3T3-L1 adipocyte-like cell

Chen-Chung Liao
contact affiliation	Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan
contact email	ccliao@ym.edu.tw
lab head
Chen-Chung Liao
contact affiliation	Proteomics Research Center,National Yang-Ming University
contact email	ccliao@ym.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031866
     1. Label: PRIDE project
     2. Name: Stomatin modulates adipogenesis through ERK pathway and regulates fatty acid uptake and lipid droplet growth