PXD031625

PXD031625 is an original dataset announced via ProteomeXchange.

Title	Localization of the priming factors CAPS1 and CAPS2 in mouse sensory neurons is determined by their N-termini
Description	Both paralogs of the calcium-dependent activator protein for secretion (CAPS) are required for exocytosis of synaptic vesicles (SVs) and large dense core vesicles (LDCVs). Despite approximately 80% sequence identity, CAPS1 and CAPS2 have distinct functions in promoting exocytosis of SVs and LDCVs in dorsal root ganglion (DRG) neurons. However, the molecular mechanisms underlying these differences remain enigmatic. In this study, we applied high- and super-resolution imaging techniques to systematically assess the subcellular localization of CAPS paralogs in DRG neurons deficient in both CAPS1 and CAPS2. CAPS1 was found to be more enriched at the synapses. Using - in-depth sequence analysis, we identified a unique CAPS1 N-terminal sequence, which we introduced into CAPS2. This CAPS1/2 chimera reproduced the pre-synaptic localization of CAPS1 and partially rescued synaptic transmission in neurons devoid of CAPS1 and CAPS2. Using immunoprecipitation combined with mass spectrometry, we identified CAPS1-specific interaction partners that could be responsible for its pre-synaptic enrichment. Taken together, these data suggest an important role of the CAPS1-N terminus in the localization of the protein at pre-synapses.
HostingRepository	PRIDE
AnnounceDate	2022-04-01
AnnouncementXML	Submission_2022-04-01_02:37:13.071.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031625
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Claudia Fecher-Trost
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	amidated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-11 06:39:13	ID requested
⏵ 1	2022-04-01 02:37:13	announced

Dataset with its publication pending

submitter keyword: Dorsal root ganglion, cellular localization, intracellular trafficking, synapse, active Zone, nano LC MS

Ute Becherer
contact affiliation	Department of Cellular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Homburg, Germany
contact email	Ute.Becherer@uks.eu
lab head
Claudia Fecher-Trost
contact affiliation	PZMS
contact email	claudia.fecher-trost@uks.eu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031625
     1. Label: PRIDE project
     2. Name: Localization of the priming factors CAPS1 and CAPS2 in mouse sensory neurons is determined by their N-termini