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PXD031566

PXD031566 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFunctional protein composition in femoral glands of sand lizards (Lacerta agilis)
DescriptionProteins are ubiquitous macromolecules displaying a vast repertoire of chemical and enzymatic functions making them suitable candidates for chemosignals used in intraspecific communication. Proteins are present in skin gland secretions of vertebrates but their identity, and especially, their functions, remain largely unknown. Many species of lizards possess femoral glands, i.e. epidermal organs primarily involved in the production and secretion of chemosignals playing a pivotal role in mate choice and intrasexual communication. The lipophilic fraction of femoral glands has been well studied in lizards. In contrast, proteins have been the focus of only a handful of investigations. Here, we study the identity, inter-individual expression patterns and functionality of proteins present in femoral glands of sand lizards (Lacerta agilis) by applying mass-spectrometry proteomics. Our results showed that the total number of proteins varied substantially among individuals. None of the identified femoral gland proteins could be directly linked to chemical communication in lizards, although this result hinges on protein annotation in databases in which squamate semiochemicals are poorly represented. In contrast to our expectations, proteins consistently expressed across individuals were related to immune system, antioxidant activity and lipid metabolism as the main functions, adding support to the hypothesis that proteins in reptilian epidermal glands have other functions besides chemical communication. Interestingly, we found that major histocompatibility complex class I (MHC) expression is enriched in femoral gland secretions. Previously, MHC was hypothesized to have been coopted to serve a semiochemical function in sand lizards, specifically in partner recognition. We speculate with the possibility that MHC proteins could be linked to semiochemical function in sand lizards.
HostingRepositoryMassIVE
AnnounceDate2022-04-06
AnnouncementXMLSubmission_2022-04-06_01:06:57.211.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUrszula Jankowska
SpeciesList scientific name: Lacerta agilis; common name: Zauneidechse; NCBI TaxID: 80427;
ModificationListOxidation; Carbamidomethyl; Acetyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-02-10 00:55:15ID requested
12022-04-06 01:06:57announced
Publication List
no publication
Keyword List
submitter keyword: Femoral glands, Inter-individual variation, Lacertidae, iBAQ
Contact List
Alejandro Ibanez
contact affiliationInstitute of Zoology and Biomedical Research, Jagiellonian University
contact emailalejandro.ibanez@uj.edu.pl
lab head
Urszula Jankowska
contact affiliationJagiellonian University
contact emailurszula.jankowska@uj.edu.pl
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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