PXD031490

PXD031490 is an original dataset announced via ProteomeXchange.

Title	Cyclodepsipeptides, solonamides, influence motility in the native producer Photobacterium galatheae S2753
Description	The marine bacterium Photobacterium galatheae S2753 produces a group of cyclodepsipeptides, solonamides, which impede the virulence but not the survival of Staphylococcus aureus. Besides the invaluable antivirulence activity, little is known about the biosynthesis and physiological function of solonamides in the native producer. This study generated a solonamides-deficient mutant, Δsol, by in-frame deleting the sol gene, thereby identifying the core gene for solonamides biosynthesis. By annotation from antiSMASH, the biosynthetic pathway of solonamides in S2753 was also proposed. Mass spectrometry analysis of cell extracts found that deficiency of solonamides production influenced the production of several novel lipids, but not the overall secondary metabolite profile. Physiological comparison between Δsol and wild-type S2753 demonstrated that growth dynamics and biofilm formation of both strains were similar; however, Δsol displayed reduced swimming rings as compared to the wild-type. The swimming ring size of Δsol was restored to the level of wild-type strain by adding solonamide B, indicating that solonamide B influences regulate the swimming behavior of P. galatheae S2753. Proteomic analysis of the Δsol and wild-type found that eliminating solonamides influenced many cellular processes, including swimming-related proteins and that solonamides trigger the motility of S2753 likely by adjusting the cellular cyclic di-GMP concentration. In conclusion, our results revealed the biosynthetic pathway of solonamides and their ecological benefits to P. galatheae S2753 by enhancing the swimming velocity, likely by altering the motile physiology.
HostingRepository	PRIDE
AnnounceDate	2022-08-17
AnnouncementXML	Submission_2022-08-17_12:08:59.166.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shengda Zhang
SpeciesList	scientific name: Photobacterium galatheae; NCBI TaxID: 1654360;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-02-07 08:25:44	ID requested
⏵ 1	2022-08-17 12:08:59	announced

Dataset with its publication pending

submitter keyword: Solonamides, Photobacterium galatheae,LC-MS, proteome, secretome

Lone Gram
contact affiliation	Department of Bioengineering, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark;
contact email	gram@bio.dtu.dk
lab head
Shengda Zhang
contact affiliation	DTU Bioengineering
contact email	shez@dtu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031490
     1. Label: PRIDE project
     2. Name: Cyclodepsipeptides, solonamides, influence motility in the native producer Photobacterium galatheae S2753