PXD031408 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity |
Description | Sulfs represent a class of unconventional sulfatases, which differ from all other members of the sulfatase family by their structures, catalytic features and biological functions. Through their specific endosulfatase activity in extracellular milieu, Sulfs provide an original post-synthetic regulatory mechanism for heparan sulfate complex polysaccharides and have been involved in multiple physiopathological processes, including cancer. However, Sulfs remain poorly characterized enzymes, with major discrepancies regarding their in vivo functions. Here we show that human Sulf-2 (HSulf-2) features a unique polysaccharide post-translational modification. We identified a chondroitin/dermatan sulfate glycosaminoglycan (GAG) chain, attached to the enzyme substrate binding domain. We found that this GAG chain affects enzyme/substrate recognition and tunes HSulf-2 activity in vitro and in vivo using a mouse model of tumorigenesis and metastasis. In addition, we showed that mammalian hyaluronidase acted as a promoter of HSulf-2 activity by digesting its GAG chain. In conclusion, our results highlight HSulf-2 as a unique proteoglycan enzyme and its newly-identified GAG chain as a critical non-catalytic modulator of the enzyme activity. These findings contribute in clarifying the conflicting data on the activities of the Sulfs and introduce a new paradigm into the study of these enzymes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-18 |
AnnouncementXML | Submission_2022-03-18_08:13:30.326.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Proteomics Core Facility |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-02-03 07:31:23 | ID requested | |
⏵ 1 | 2022-03-18 08:13:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: sulfatase, enzyme, glycosaminoglycan, proteoglycan, post-translational modifications |
Contact List
Romain R. Vivès |
contact affiliation | Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France |
contact email | romain.vives@ibs.fr |
lab head | |
Proteomics Core Facility |
contact affiliation | SAMBIO Core Facilities, Sahgrenska Academy, University of Gothenburg |
contact email | gupcf@outlook.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031408
- Label: PRIDE project
- Name: Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity