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PXD031335

PXD031335 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleParamecium Polycomb Repressive Complex 2 physically interacts with the small RNA binding PIWI protein to repress transposable elements
DescriptionPolycomb Repressive Complex 2 (PRC2) maintains transcriptionally silent genes in a repressed state via deposition of histone H3 K27 trimethyl (me3) marks. PRC2 has also been implicated in silencing transposable elements (TEs) yet how PRC2 is targeted to TEs remains unclear. To address this question, we performed tandem affinity purification combined with mass spectrometry and identified proteins that physically interact with the Paramecium Enhancer-of-zeste Ezl1 enzyme, which catalyzes H3K9me3 and H3K27me3 deposition at TEs. We show that the Paramecium PRC2 core complex comprises four subunits, each required in vivo for catalytic activity. We also identify PRC2 cofactors, including the RNA interference (RNAi) effector Ptiwi09, which are necessary to target H3K9me3 and H3K27me3 to TEs. We find that the physical interaction between PRC2 and the RNAi pathway is mediated by a RING finger protein and that small RNA recruitment of PRC2 to TEs is analogous to the small RNA recruitment of H3K9 methylation SU(VAR)3-9 enzymes.
HostingRepositoryPRIDE
AnnounceDate2022-04-06
AnnouncementXMLSubmission_2022-04-06_01:31:09.138.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaurent Lignières
SpeciesList scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-30 11:06:51ID requested
12022-04-06 01:31:09announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Paramecium, tetraurelia, small RNA, PIWI, Polycomb Repressive Complex
Contact List
Guillaume Chevreux
contact affiliationProteomic facility, Institut Jacques Monod UMR7592 (CNRS & Université de Paris)
contact emailguillaume.chevreux@ijm.fr
lab head
Laurent Lignières
contact affiliationProteomic facility of Institut Jacques Monod (UMR7592) - CNRS & Université de Paris
contact emaillaurent.lignieres@ijm.fr
dataset submitter
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Dataset FTP location
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