PXD031335 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Paramecium Polycomb Repressive Complex 2 physically interacts with the small RNA binding PIWI protein to repress transposable elements |
Description | Polycomb Repressive Complex 2 (PRC2) maintains transcriptionally silent genes in a repressed state via deposition of histone H3 K27 trimethyl (me3) marks. PRC2 has also been implicated in silencing transposable elements (TEs) yet how PRC2 is targeted to TEs remains unclear. To address this question, we performed tandem affinity purification combined with mass spectrometry and identified proteins that physically interact with the Paramecium Enhancer-of-zeste Ezl1 enzyme, which catalyzes H3K9me3 and H3K27me3 deposition at TEs. We show that the Paramecium PRC2 core complex comprises four subunits, each required in vivo for catalytic activity. We also identify PRC2 cofactors, including the RNA interference (RNAi) effector Ptiwi09, which are necessary to target H3K9me3 and H3K27me3 to TEs. We find that the physical interaction between PRC2 and the RNAi pathway is mediated by a RING finger protein and that small RNA recruitment of PRC2 to TEs is analogous to the small RNA recruitment of H3K9 methylation SU(VAR)3-9 enzymes. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-06 |
AnnouncementXML | Submission_2022-04-06_01:31:09.138.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laurent Lignières |
SpeciesList | scientific name: Paramecium tetraurelia; NCBI TaxID: 5888; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-30 11:06:51 | ID requested | |
⏵ 1 | 2022-04-06 01:31:09 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Paramecium, tetraurelia, small RNA, PIWI, Polycomb Repressive Complex |
Contact List
Guillaume Chevreux |
contact affiliation | Proteomic facility, Institut Jacques Monod UMR7592 (CNRS & Université de Paris) |
contact email | guillaume.chevreux@ijm.fr |
lab head | |
Laurent Lignières |
contact affiliation | Proteomic facility of Institut Jacques Monod (UMR7592) - CNRS & Université de Paris |
contact email | laurent.lignieres@ijm.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031335
- Label: PRIDE project
- Name: Paramecium Polycomb Repressive Complex 2 physically interacts with the small RNA binding PIWI protein to repress transposable elements