PXD031331

PXD031331 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.
Description	Background: Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives: In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods: Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (Tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results: In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions: We screened 1645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_04:53:03.984.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hongxia pei
SpeciesList	scientific name: Capsicum annuum; NCBI TaxID: 4072;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-30 11:04:51	ID requested
⏵ 1	2022-05-19 04:53:04	announced

Dataset with its publication pending

submitter keyword: Male sterility； proteomic；Parallel reaction monitoring (PRM)；Phytohormone； Pepper (Capsicum annuum L.)

Hongxia pei
contact affiliation	College of Forestry and Horticulture, Xinjiang Agricultural University；Institute of Horticulture Crops, Ningxia Academy of Agriculture and Forestry Sciences；
contact email	hongxiapei2021@163.com
lab head
hongxia pei
contact affiliation	Institute of Horticulture Crops, Ningxia Academy of Agriculture and Forestry Sciences
contact email	hongxiapei2021@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031331
     1. Label: PRIDE project
     2. Name: Proteomic analysis of differential anther development from sterile/fertile lines in Capsicum annuum L.