PXD031224

PXD031224 is an original dataset announced via ProteomeXchange.

Title	RIPK3 is released into Extracellular Vesicles during Necroptosis through a Rab27 independent mechanism
Description	Extracellular vesicles (EVs) are important mechanisms used by cells to release biomolecules. A common necroptosis effector— mixed lineage kinase like (MLKL)--- was recently found to participate in the biogenesis of small and large EVs independent of its function in necroptosis. The objective of the current study is to gain mechanistic insights into EV biogenesis during necroptosis. We performed mass spectrometry-based proteomics on EVs released by healthy or necroptotic cells. Necroptosis increased the number of EVs released and altered the protein contents within the EVs. Comparing to EVs released by healthy cells, EVs released during necroptosis contained markedly higher number of unique proteins. Receptor interacting protein kinase 3 (RIPK3) and MLKL were among the proteins enriched in EVs released during necroptosis. Further, MEFs derived from mice deficient of Rab27a and Rab27b showed diminished basal EV release but responded to necroptosis with enhanced EV biogenesis as the wildtype MEFs. In contrast, necroptosis-associated EVs was sensitive to Ca2+ depletion or lysosomal disruption. Neither treatments affected the RIPK3-mediated MLKL phosphorylation. Our data suggests that necroptosis switches EV biogenesis from a Rab27a/b dependent mechanism to a lysosomal mediated mechanism.
HostingRepository	PRIDE
AnnounceDate	2022-08-14
AnnouncementXML	Submission_2022-08-14_10:50:06.651.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kyle brown
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-25 00:49:09	ID requested
⏵ 1	2022-08-14 10:50:07	announced

Dataset with its publication pending

submitter keyword: RIPK3, lysosomal exocytosis, MLKL, Necroptosis, Proteomics, SEVs

Bo Liu
contact affiliation	SMPH/SURGERY/VASCULAR SURGERY
contact email	liub@surgery.wisc.edu
lab head
kyle brown
contact affiliation	uw madison
contact email	kbrown33@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031224

PRIDE project URI

• PRIDE
  1. PXD031224
     1. Label: PRIDE project
     2. Name: RIPK3 is released into Extracellular Vesicles during Necroptosis through a Rab27 independent mechanism