PXD031063 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization |
Description | The phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enigmatic. Auxin Binding Protein 1 (ABP1) has been contested as an auxin receptor candidate since decades. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at the acidic pH typical for the apoplast. ABP1 and its plasma membrane-localized partner, Transmembrane Kinase 1 (TMK1) are required for auxin-induced phosphorylation of about thousand proteins. Loss-of-function abp1 alleles but not complemented lines show defects in in vitro shoot regeneration and formation of auxin-transporting channels for vasculature formation and regeneration. These results support a role of ABP1-TMK1 in cell surface auxin perception mediating global auxin phospho-response and regenerative development. |
HostingRepository | PRIDE |
AnnounceDate | 2022-08-25 |
AnnouncementXML | Submission_2022-08-25_03:50:37.739.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mark Roosjen |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-19 02:36:06 | ID requested | |
⏵ 1 | 2022-08-25 03:50:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Auxin ABP1 TMK1 phosphoproteomics |
Contact List
Prof.Dr. Dolf Weijers |
contact affiliation | Department head |
contact email | dolf.weijers@wur.nl |
lab head | |
Mark Roosjen |
contact affiliation | Wageningen University and Research |
contact email | mark.roosjen@wur.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD031063
- Label: PRIDE project
- Name: ABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization