<<< Full experiment listing

PXD031063

PXD031063 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleABP1-TMK auxin perception mediates rapid phosphorylation for regeneration and auxin canalization
DescriptionThe phytohormone auxin triggers transcriptional reprograming utilizing a well-characterized nuclear perception machinery. In contrast, mechanisms underlying other auxin effects, such as auxin feed-back on its transport, rapid regulation of ion fluxes or ultrafast global phospho-response, remain enigmatic. Auxin Binding Protein 1 (ABP1) has been contested as an auxin receptor candidate since decades. Here we show that a fraction of Arabidopsis thaliana ABP1 is secreted and binds auxin specifically at the acidic pH typical for the apoplast. ABP1 and its plasma membrane-localized partner, Transmembrane Kinase 1 (TMK1) are required for auxin-induced phosphorylation of about thousand proteins. Loss-of-function abp1 alleles but not complemented lines show defects in in vitro shoot regeneration and formation of auxin-transporting channels for vasculature formation and regeneration. These results support a role of ABP1-TMK1 in cell surface auxin perception mediating global auxin phospho-response and regenerative development.
HostingRepositoryPRIDE
AnnounceDate2022-08-25
AnnouncementXMLSubmission_2022-08-25_03:50:37.739.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMark Roosjen
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListphosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-19 02:36:06ID requested
12022-08-25 03:50:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Auxin ABP1 TMK1 phosphoproteomics
Contact List
Prof.Dr. Dolf Weijers
contact affiliationDepartment head
contact emaildolf.weijers@wur.nl
lab head
Mark Roosjen
contact affiliationWageningen University and Research
contact emailmark.roosjen@wur.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD031063
PRIDE project URI
Repository Record List
[ + ]