PXD031054 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics and in-cell cross-linking reveal cellular reorganisation during early neuronal differentiation of SH-SY5Y cells |
Description | The proteomes of undifferentiated and differentiated SH-SY5Y cells are characterised and compared. For this, neuronal differentiation using retinoic acid (RA) or a combination of RA and phorbol-12-myristat-13-acetate (RA/PMA) was explored. An MS-based label-free quantification approach is applied to identify changes in the protein expression, the as proteins’ subcellular localisation abundance as well as their association with enriched KEGG pathways. By employing formaldehyde cross-linking insights into protein interaction networks of undifferentiated as well as RA- and RA/PMA-differentiated neurons are obtained. The analyses provided insights into the proteomes of undifferentiated and differentiated SH-SY5Y cells and suggest structural rearrangements, for instance, of the actin network during neuronal differentiation. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-08 |
AnnouncementXML | Submission_2022-04-08_08:41:09.594.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marie Barth |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-18 02:32:12 | ID requested | |
1 | 2022-04-08 03:12:33 | announced | |
⏵ 2 | 2022-04-08 08:41:09 | announced | 2022-04-08: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, chemical cross-linking, mass spectrometry, protein networks, actin, SH-SY5Y |
Contact List
Carla Schmidt |
contact affiliation | Interdisciplinary research center HALOmem, Institute of Biochemistry and Biotechnology, Charles Tanford Protein Center, Martin Luther University Halle-Wittenberg, Halle, Germany |
contact email | carla.schmidt@biochemtech.uni-halle.de |
lab head | |
Marie Barth |
contact affiliation | HALOmem, Martin Luther University Halle-Wittenberg |
contact email | marie.barth@bct.uni-halle.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD031054 |
PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD031054
- Label: PRIDE project
- Name: Quantitative proteomics and in-cell cross-linking reveal cellular reorganisation during early neuronal differentiation of SH-SY5Y cells