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PXD030782

PXD030782 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeroxisome-derived hydrogen peroxide can modulate the sulfenylation profiles of key redox signaling proteins in Flp-In T-REx 293 cells
DescriptionEver since the first characterization of peroxisomes, a central theme has been their involvement in cellular hydrogen peroxide (H2O2) metabolism. While the reputation of H2O2 drastically changed from an exclusively toxic molecule to a signaling messenger, the regulatory role of peroxisomes in these signaling events is still largely underappreciated. This is mainly because the number of known protein targets of peroxisome-derived H2O2 is rather limited and testing of specific targets is predominantly based on knowledge previously gathered in related fields of research. To gain a broader and more systematic insight into the role of peroxisomes in redox signaling, new approaches are urgently needed. In this study, we have combined a previously developed Flp-In T-REx 293 cell system in which peroxisomal H2O2 production can be modulated with a yeast AP-1-like-based sulfenome mining strategy to inventory protein thiol targets of peroxisome-derived H2O2 in different subcellular compartments. Using this approach, we were able to identify specific and common targets of peroxisome-derived and exogenous H2O2 in peroxisomes, the cytosol, and mitochondria. We also observed that the sulfenylation kinetics profiles of key targets (e.g., PRDX1, ANXA2, and TUBA1C) belonging to different protein families (e.g., antioxidants, annexins, and tubulins) can vary considerably. In addition, we obtained compelling but indirect evidence that peroxisome-derived H2O2 may oxidize at least some of its targets (e.g., transcription factors) through a redox relay mechanism. In conclusion, given that sulfenic acids function as key intermediates in H2O2 signaling, the findings presented in this study provide initial but critical insight into how peroxisomes may be integrated into the cellular H2O2 signaling network.
HostingRepositoryPRIDE
AnnounceDate2022-05-10
AnnouncementXMLSubmission_2022-05-10_07:25:29.927.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD030782
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCelien Lismont
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; N-methylmaleimide modified L-cysteine; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-01-06 01:50:36ID requested
12022-05-10 07:25:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Peroxisome
hydrogen peroxide
cysteine thiol group
YAP1C-based sulfenome mining
peroxiredoxin
mitochondrion
Contact List
Marc Fransen
contact affiliationDepartment of cellular and molecular medicine, Laboratory of peroxisome biology and intracellular communication, KU Leuven, Belgium
contact emailmarc.fransen@kuleuven.be
lab head
Celien Lismont
contact affiliationKU Leuven
contact emailcelien.lismont@kuleuven.be
dataset submitter
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Dataset FTP location
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