PXD030782 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peroxisome-derived hydrogen peroxide can modulate the sulfenylation profiles of key redox signaling proteins in Flp-In T-REx 293 cells |
Description | Ever since the first characterization of peroxisomes, a central theme has been their involvement in cellular hydrogen peroxide (H2O2) metabolism. While the reputation of H2O2 drastically changed from an exclusively toxic molecule to a signaling messenger, the regulatory role of peroxisomes in these signaling events is still largely underappreciated. This is mainly because the number of known protein targets of peroxisome-derived H2O2 is rather limited and testing of specific targets is predominantly based on knowledge previously gathered in related fields of research. To gain a broader and more systematic insight into the role of peroxisomes in redox signaling, new approaches are urgently needed. In this study, we have combined a previously developed Flp-In T-REx 293 cell system in which peroxisomal H2O2 production can be modulated with a yeast AP-1-like-based sulfenome mining strategy to inventory protein thiol targets of peroxisome-derived H2O2 in different subcellular compartments. Using this approach, we were able to identify specific and common targets of peroxisome-derived and exogenous H2O2 in peroxisomes, the cytosol, and mitochondria. We also observed that the sulfenylation kinetics profiles of key targets (e.g., PRDX1, ANXA2, and TUBA1C) belonging to different protein families (e.g., antioxidants, annexins, and tubulins) can vary considerably. In addition, we obtained compelling but indirect evidence that peroxisome-derived H2O2 may oxidize at least some of its targets (e.g., transcription factors) through a redox relay mechanism. In conclusion, given that sulfenic acids function as key intermediates in H2O2 signaling, the findings presented in this study provide initial but critical insight into how peroxisomes may be integrated into the cellular H2O2 signaling network. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-10 |
AnnouncementXML | Submission_2022-05-10_07:25:29.927.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD030782 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Celien Lismont |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; N-methylmaleimide modified L-cysteine; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-01-06 01:50:36 | ID requested | |
⏵ 1 | 2022-05-10 07:25:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Peroxisome |
hydrogen peroxide |
cysteine thiol group |
YAP1C-based sulfenome mining |
peroxiredoxin |
mitochondrion |
Contact List
Marc Fransen |
contact affiliation | Department of cellular and molecular medicine, Laboratory of peroxisome biology and intracellular communication, KU Leuven, Belgium |
contact email | marc.fransen@kuleuven.be |
lab head | |
Celien Lismont |
contact affiliation | KU Leuven |
contact email | celien.lismont@kuleuven.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030782 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030782
- Label: PRIDE project
- Name: Peroxisome-derived hydrogen peroxide can modulate the sulfenylation profiles of key redox signaling proteins in Flp-In T-REx 293 cells