PXD030663 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cell-type-specific labeling and profiling of glycans in living mice |
Description | Metabolic labeling of glycans with clickable unnatural sugars has enabled glycan imaging and glycoproteomics in mixed cell populations. However, in vivo labeling and profiling of cell-type-specific glycans remains challenging. Here, we develop genetically encoded metabolic glycan labeling (GeMGL), a cell-type-specific strategy based on a bump-hole pair of an unnatural sugar and its matching engineered enzyme. N pentynylacetylglucosamine (GlcNAl) serves as a bumped analog of N acetylglucosamine (GlcNAc) that can be specifically incorporated into glycans of cells expressing an engineered mutant of UDP-GlcNAc pyrophosphorylase, AGX2F383G. GeMGL with the 1,3-di-O-propionylated GlcNAl (1,3-Pr2GlcNAl) and AGX2F383G pair is demonstrated in cell co-cultures, and used for specific labeling of glycans in mouse xenograft tumors. By generating a transgenic mouse line with AGX2F383G expressed under cardiomyocyte-specific promoter, we perform specific imaging of cardiomyocyte glycans in the heart and identified 582 cardiomyocyte O-GlcNAcylated proteins with no interference from other cardiac cell types. GeMGL will facilitate cell-type-specific glycoproteomics in various tissues and disease models. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-28 |
AnnouncementXML | Submission_2022-02-28_12:42:32.799.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Qitao Song |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-(N-acetylamino)glucosyl-L-cysteine; O-(N-acetylamino)glucosyl-L-threonine; O-(N-acetylamino)glucosyl-L-serine |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-30 03:22:03 | ID requested | |
⏵ 1 | 2022-02-28 12:42:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Glycan, cell-type-specific, metabolic glycan labeling |
Contact List
Xing Chen |
contact affiliation | College of Chemistry and Molecular Engineering, Peking University, Beijing, China |
contact email | xingchen@pku.edu.cn |
lab head | |
Qitao Song |
contact affiliation | Peking University |
contact email | sqtwgq@sina.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030663
- Label: PRIDE project
- Name: Cell-type-specific labeling and profiling of glycans in living mice