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PXD030619

PXD030619 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMass Spectrometry Uncovers Protein Interactions and Stoichiometry of Intermediates and Sub-complexes for SNARE Complex Assembly
DescriptionThe neuronal SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 anchored to the presynaptic membrane. It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters into the synaptic cleft. While the general sequence of SNARE complex formation is well-established, our knowledge on possible intermediates is still incomplete. We, therefore, follow the step-wise assembly of the SNARE complex and target individual SNAREs, binary sub-complexes, the ternary SNARE complex as well as interactions with Complexin-1. Using native mass spectrometry, we identify the stoichiometry of preferred sub-complexes and monitor oligomerisation of various assemblies. Importantly, we find that interactions with Complexin-1 reduce self-association of the ternary SNARE complex. Chemical cross-linking provides detailed insights into these interactions suggesting a role during membrane fusion. Taken together, we unravel possible intermediates and preferred sub-complexes and compile a road map of SNARE complex assembly including regulation by Complexin-1.
HostingRepositoryPRIDE
AnnounceDate2023-01-25
AnnouncementXMLSubmission_2023-01-25_11:38:19.293.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarieAlfes
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-23 00:34:28ID requested
12023-01-25 11:38:19announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: native mass spectrometry, chemical cross-linking,SNARE complex, Complexin-1, signal transmission in neurons
Contact List
CarlaSchmidt
contact affiliationInterdisciplinary Research Centre HALOmem, Charles Tanford Protein Centre, Institute of Biochemistry and Biotechnology, Martin-Luther University Halle-Wittenberg, Halle, Germany
contact emailcarla.schmidt@biochemtech.uni-halle.de
lab head
MarieAlfes
contact affiliationHALOmem, Martin Luther University Halle-Wittenberg
contact emailmarie.barth@bct.uni-halle.de
dataset submitter
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Dataset FTP location
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