PXD030334

PXD030334 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Human Conjunctival Cells and Tears Collected with Schirmer Strips Using TimsTOF Pro
Description	This study aimed to investigate the human tear proteins extracted from whole (W) Schirmer strips (ScS) and their two parts, the bulb (B) and the rest of the strip (R) with a comprehensive proteomic approach using a trapped ion mobility mass spectrometer, the timsTOF Pro. Eight ScS were collected from two healthy subjects at 4 different visits to be separated into three batches: 4W, 4B and 4R. In total, 1582 proteins were identified in the W, B and R batches. Among all iden-tified proteins, binding proteins (43.4%) and those with catalytic activity (42.2%) constituted more than 80% of the molecular functions. The most represented biological processes were cel-lular processes (31.2%), metabolic processes (20.8%) and biological regulation (13.1%). Enzymes were the most represented protein class (41 %), consisting mainly of hydrolases (47.5%), oxi-doreductases (22.1%) and transferases (16.7%). The bulb (B), which is in contact with the conjunc-tiva, might collect both tear and cell proteins and therefore promote identification of more pro-teins. Processing B and R separately before MS analysis, combined with the high data acquisition speed and the addition of ion-mobility-based separation in timsTOF Pro, can bring a new dimension to biomarker investigations of a limited sample such as tear fluid.
HostingRepository	PRIDE
AnnounceDate	2022-01-27
AnnouncementXML	Submission_2022-01-27_02:29:02.639.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Solenne Chardonnet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-12 13:58:51	ID requested
⏵ 1	2022-01-27 02:29:02	announced

Dataset with its publication pending

submitter keyword: Tears, Schirmer strips, conjunctival cells, proteomics, timsTOF Pro

Solenne Chardonnet
contact affiliation	Sorbonne Université, UMS PASS, Plateforme P3S
contact email	solenne.chardonnet@sorbonne-universite.fr
lab head
Solenne Chardonnet
contact affiliation	Sorbonne Universite UMS PASS - P3S
contact email	solenne.chardonnet@sorbonne-universite.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD030334

PRIDE project URI

• PRIDE
  1. PXD030334
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Human Conjunctival Cells and Tears Collected with Schirmer Strips Using TimsTOF Pro