PXD030201
PXD030201 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Oxidation of whey proteins during thermal treatment characterized by a site-specific LC-MS/MS-based proteomic approach |
| Description | Thermal treatment is often employed in food processing to tailor product properties by manipulating ingredient functionality. However, due to the presence of oxidants in many food products, these elevated temperatures may accelerate oxidation and lead to the loss of nutrients. In the present study, oxidation of different whey protein systems (pure alpha-lactalbumin (a-LA), pure beta-lactoglobulin (b-LG), a mix of a-LA and b-LG (as a whey model) and a commercial whey protein isolate (WPI)) was investigated during heat treatment at 60-90 degreeC and a UHT-like condition by LC-MS-based proteomic analysis. The relative modification levels of each oxidation site were calculated and compared among different heat treatments and sample systems. The temperature- and time-dependent oxidation was detectable in protein systems after heating at and higher than 90 degreeC, while the extent of oxidation decreased with increasing complexity of the system (a-LA > b-LG > whey model > WPI). Trp residues in a-LA were found to be the amino acid residues most prone to oxidation during heat treatment. In b-LG-containing protein systems, Cys residues were suggested to scavenge most of the reactive oxidants and undergo oxidation-mediated disulfide rearrangement. The rearranged disulfide bonds contributed to protein aggregation (as confirmed by SDS-PAGE analysis), which was suggested to provide further physical protection against oxidation. However, no significant loss of amino acid residues was detected in the heated protein systems after acidic hydrolysis followed by HPLC analysis, which shows only a minor effect of heat treatment on protein oxidation in these pure protein systems. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-12-06 |
| AnnouncementXML | Submission_2021-12-06_04:09:06.214.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chengkang |
| SpeciesList | scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913; |
| ModificationList | Oxidation; Carbamidomethyl; Dioxidation; Trioxidation; oxidation of tyrosine to L-2',4',5'-topaquinone; Trp->Hydroxykynurenin; unknown modification; unknown modification; unknown modification; unknown modification; L-kynurenine; unknown modification |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-12-06 02:38:24 | ID requested | |
| ⏵ 1 | 2021-12-06 04:09:06 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Protein oxidation, cysteine, tryptophan, disulfide cross-link, protein aggregation, antioxidant |
Contact List
| Marianne Nissen Lund | |
|---|---|
| contact affiliation | Department of Food Science, University of Copenhagen |
| contact email | mnl@food.ku.dk |
| lab head | |
| Chengkang | |
| contact affiliation | University of Copenhagen |
| contact email | licky_lck@hotmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088520/ |
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