PXD030185

PXD030185 is an original dataset announced via ProteomeXchange.

Title	The FtsH-inactive Protein TSL2/FtsHi5 Is Required for Chloroplast Development and Proteostasis at Low Ambient Temperature in Arabidopsis thaliana
Description	Chloroplasts are indispensable for higher plants. The growth and development of plants are very sensitive to environmental temperature changes, and chloroplast development is also regulated by adverse environmental temperatures. However, the molecular mechanism of how plants coordinate chloroplast development and environmental temperature changes remains largely unknown. Here, a temperature-conditioned chloroplast development defect mutant tsl2 (thermo-sensitive mutant in leaf color 2) of Arabidopsis was obtained through forward genetic screening. The tsl2 mutant showed a weak yellowing phenotype at 22 °C which was more pronounced when the growth temperature was reduced to 16 °C, and was similar to that of the wild type when the temperature was increased to 29 °C. Genomic resequencing analysis revealed that TSL2 gene encodes the FtsHi5 which was shown to function in chloroplast protein import. Genetic complementation analysis showed that TSL2 rescued the chlorophyll content and thylakoid development defects in tls2 mutants at 16 °C. Quantitative mass spectrometry analysis with Tandem Mass Tag (TMT) isobaric labeling revealed broad changes in the proteome composition of tsl2 chloroplasts at low temperature, reflecting impaired chloroplast biogenesis and function. Together, our data suggest that TSL2 has a significant role in chloroplast development and proteostasis especially at low environmental temperatures.
HostingRepository	PRIDE
AnnounceDate	2022-01-21
AnnouncementXML	Submission_2022-01-20_19:45:23.618.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinyu Li
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-05 04:50:13	ID requested
⏵ 1	2022-01-20 19:45:24	announced

Dataset with its publication pending

submitter keyword: FtsH, Chloroplast biogenesis, Chloroplast Proteome, Arabidopsis thaliana, TMT

Jianxiang Liu
contact affiliation	State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310027, China.
contact email	jianxiangliu@zju.edu.cn
lab head
Jinyu Li
contact affiliation	College of Life Sciences, Zhejiang University, Hangzhou 310027, China.
contact email	lijinyu2009@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030185
     1. Label: PRIDE project
     2. Name: The FtsH-inactive Protein TSL2/FtsHi5 Is Required for Chloroplast Development and Proteostasis at Low Ambient Temperature in Arabidopsis thaliana