PXD030049

PXD030049 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis on the protective effect of eriodictyol on benzo(a)pyrene-induced Caco-2 cytotoxicity
Description	The present study was aimed to evaluate the protective effects of six different polyphenols against benzo(a)pyrene (BaP)-induced cytotoxicity in Caco-2 cells. The results shows that treatment of quinic acid, ferulic acid, homovanillic acid and BaP decreased the cell viability, whereas only co-treatment of 20 µM eriodictyol and naringenin reduced BaP-induced cytotoxicity, including cell apoptosis, cell cycle change and oxidative stress. Moreover, all results show that the inhibitory effect of eriodictyol was better than that of naringenin. Further, the potential protective mechanism of eriodictyol against BaP-induced toxicity was investigated by proteomics. We found that the genetic information processing pathway involves in 80 differentially expressed proteins (DEPs) showed the highest proportion and number of total proteins. Finally, eriodictyol inhibited BaP-induced cytotoxicity by regulating the expression of key proteins such as RPA2, SNRPA, RAD23B, NUP155 and AARS in transcription and translation. Overall, our results provide a new perspective for polyphenols inhibiting BaP-induced carcinogenesis.
HostingRepository	PRIDE
AnnounceDate	2021-12-17
AnnouncementXML	Submission_2021-12-17_02:29:02.260.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chong Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-29 09:52:11	ID requested
⏵ 1	2021-12-17 02:29:02	announced

Dataset with its publication pending

submitter keyword: benzo(a)pyrene, eriodictyol, Caco-2 cells, cytotoxicity, proteomics

Chong Wang
contact affiliation	University of Copenhagen
contact email	chong.wang@bio.ku.dk
lab head
Chong Wang
contact affiliation	Nanjing Agricultural University
contact email	dacong0623@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030049
     1. Label: PRIDE project
     2. Name: Proteomic analysis on the protective effect of eriodictyol on benzo(a)pyrene-induced Caco-2 cytotoxicity