PXD029899

PXD029899 is an original dataset announced via ProteomeXchange.

Title	Polypyrimidine Tract Binding Proteins PTBP1 and PTBP2 Interact with Distinct Proteins Under Splicing Conditions
Description	RNA binding proteins play an important role in regulating alternative pre-mRNA splicing and in turn cellular gene expression. Polypyrimidine tract binding proteins, PTBP1 and PTBP2, are paralogous RNA binding proteins that play a critical role in the process of neuronal differentiation and maturation; changes in the concentration of PTBP proteins during neuronal development direct splicing changes in many transcripts that code for proteins critical for neuronal differentiation. How the two related proteins regulate different sets of neuronal exons is unclear. The distinct splicing activities of PTBP1 and PTBP2 can be recapitulated in an in vitro splicing system with the differentially regulated N1 exon of the c-src pre-mRNA. Here, we conducted experiments under these in vitro splicing conditions to identify PTBP1 and PTBP2 interacting partner proteins.
HostingRepository	PRIDE
AnnounceDate	2022-01-20
AnnouncementXML	Submission_2022-01-19_23:54:14.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeffrey Pina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-24 06:07:44	ID requested
⏵ 1	2022-01-19 23:54:15	announced

Dataset with its publication pending

submitter keyword: PTB, nPTB, PTBP, PTBP1, PTBP2, splicing, LC-MS/MS

Niroshika Monerawila Keppetipola
contact affiliation	Department of Chemistry and Biochemistry, California State University Fullerton
contact email	nkeppetipola@fullerton.edu
lab head
Jeffrey Pina
contact affiliation	California State University Fullerton
contact email	jeffreypina@fullerton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029899
     1. Label: PRIDE project
     2. Name: Polypyrimidine Tract Binding Proteins PTBP1 and PTBP2 Interact with Distinct Proteins Under Splicing Conditions