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PXD029793

PXD029793 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSerum proteomic profile of asthmatic patients after 6 months of Benralizumab and Mepolizumab treatment
DescriptionSevere eosinophilic asthma is characterized by chronic airway inflammation, oxidative stress and elevated proinflammatory cytokines, especially IL-5. Mepolizumab and benralizumab are both humanized IgG antibodies directed against IL-5 signaling, directly acting on eosinophils count. Together with the complexity of severe asthma classification and to the patient selection for the targeted treatment, there is also the urgency to clarify the follow-up of therapy to identify biomarkers, in addition to eosinophils, for the optimal duration of treatment, persistence of effectiveness and safety. To this purpose, here we performed a follow-up study by differential proteomic analysis on serum samples after 1 month and 6 months of both therapies and sera from healthy patients. Statistical analysis by PCA and heatmap analyses were performed, and identified proteins were used for enrichment analysis by MetaCore software. Analysis highlights 82 differences among all considered conditions. In particular, 30 referred to benralizumab time points (T0, T1B, T6B) and 24 to mepolizumab time points (T0, T1M, T6M) analyses. t-SNE and heatmap analyses evidences as the differential serum protein profile at 6 months of both treatments is more similar to that of the healthy subjects. Among the identified proteins, APOAI, APOC-II and APOC-III are up-regulated principally after six months of benralizumab treatment, plasminogen is up-regulated after six months of both treatments and ceruloplasmin, up-regulated already after 1 month of benralizumab, become higher after 6 months of mepolizumab. By enrichment analysis, identified proteins were related to lipid metabolism and transport, blood coagulation and ECM remodelling.
HostingRepositoryPRIDE
AnnounceDate2022-04-01
AnnouncementXMLSubmission_2022-04-01_04:13:39.272.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLorenza Vantaggiato
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
Instrumentultraflex
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-17 23:56:37ID requested
12022-04-01 04:13:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Mepolizumab
Benralizumab
ceruloplasmin
plasminogen
ApoA1, ApoC, severe eosinophilic asthma.
Contact List
Professor Luca Bini
contact affiliationFunctional proteomis lab, Life Sciences Dept., University of Siena
contact emailluca.bini@unisi.it
lab head
Lorenza Vantaggiato
contact affiliationuniversity of siena
contact emaillorenz.vantaggiato@student.unisi.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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