PXD029396

PXD029396 is an original dataset announced via ProteomeXchange.

Title	Actinobacillus pleuropneumoniae serotype 2 exoproteome LF-LC-MS/MS
Description	Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae affects pig health status and the swine industry worldwide. Despite of the extensive number of studies focused on A. pleuropneumoniae infection and vaccine development, its exoproteome is still rather unexplored. By combining high-throughput mass spectrometry and immunoproteomic approaches, with our current work we provide an in-depth characterisation of A. pleuropneumoniae serotype 2 exoproteome. Label-free liquid chromatography - tandem mass spectrometry (LC-MS/MS) combined with a comprehensive bioinformatics analysis revealed 484 secreted proteins, of which 84 were predicted to be virulence factors and 142 to be exported via different export mechanisms. The RTX toxins ApxIIA, ApxIIIA and ApxIVA were found to be the most abundant proteins in the A. pleuropneumoniae serotype 2 exoproteome, although ApxIVA is commonly assumed to be expressed exclusively in vivo. Immunoproteomic approaches coupled to LC-MS/MS analysis allowed to portray the immunogenic proteins within the bacterial exoproteome to identify potential vaccine candidates. Using serum pools from uninfected, acutely infected and chronically infected animals, we were able to monitor the seroconversion during disease progression. Overall, our work is expected to contribute to the understanding of the complex pathogenic mechanisms and to facilitate the discovery of potential antimicrobial agents for controlling porcine pleuropneumonia.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:29.396.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stelli Stancheva
SpeciesList	scientific name: Actinobacillus pleuropneumoniae serovar 2; NCBI TaxID: 34063;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-28 06:41:30	ID requested
1	2022-10-13 07:07:47	announced
⏵ 2	2023-11-14 08:54:29	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: porcine pleuropneumonia, exoproteome,Actinobacillus pleuropneumoniae

Monika Ehling-Schulz
contact affiliation	Institute of Microbiology Department of Pathobiology University of Veterinary Medicine Veterinaerplatz 1 1210 Vienna Austria
contact email	Monika.Ehling-Schulz@vetmeduni.ac.at
lab head
Stelli Stancheva
contact affiliation	University of Veterinary Medicine, Vienna, Austria
contact email	stelli.stancheva@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD029396

PRIDE project URI

• PRIDE
  1. PXD029396
     1. Label: PRIDE project
     2. Name: Actinobacillus pleuropneumoniae serotype 2 exoproteome LF-LC-MS/MS