PXD029270 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cysteine 253 of UCP1 regulates energy expenditure and adipose tissue inflammation |
Description | Uncoupling protein 1 (UCP1) is thought to be a major regulator of whole-body energy expenditure and metabolic homeostasis. However, the widely employed UCP1 loss of function model has recently been shown to have destructive effects on the entire electron transport chain of thermogenic fat. As such, the role of UCP1 in metabolic regulation in vivo remains unclear. We recently identified cysteine-253 as an allosteric site on UCP1 that elevates protein activity upon covalent modification. Here we examine the physiological importance of this site through the generation of a UCP1 cysteine-253 null mouse (UCP1 C253A), the first genetic model for selective disruption of UCP1 in vivo. UCP1 C253A mice exhibit significantly compromised thermogenic responses but display no measurable effect on fat accumulation in an obesogenic environment. Unexpectedly, instead we find that lack of cysteine-253 results in substantial immune cell infiltration and inflammatory pathology in adipose tissues of male, but not female mice. Together, our results establish the UCP1 cysteine-253 activation site as a regulator of acute thermogenesis and sex-dependent adipose tissue inflammation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-15 |
AnnouncementXML | Submission_2021-12-15_03:06:33.374.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Haopeng Xiao |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue |
Instrument | Orbitrap Eclipse; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-21 05:53:03 | ID requested | |
⏵ 1 | 2021-12-15 03:06:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Thermogenesis, UCP1, inflammation, adipose, sex-dependent |
Contact List
Edward Chouchani |
contact affiliation | 1 Department of Cancer Biology, Dana–Farber Cancer Institute, Boston, MA, USA. 2 Department of Cell Biology, Harvard Medical School, Boston, MA, USA. |
contact email | EdwardT_Chouchani@DFCI.HARVARD.EDU |
lab head | |
Haopeng Xiao |
contact affiliation | Dana-Farber Cancer Institute/Harvard Medical School |
contact email | haopeng_xiao@dfci.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029270
- Label: PRIDE project
- Name: Cysteine 253 of UCP1 regulates energy expenditure and adipose tissue inflammation