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PXD029205

PXD029205 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic dataset of the moss Physcomitrium patens SMG1 KO mutant line
DescriptionHere, we provide an isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomic dataset of the moss Physcomitrium patens smg1 knockout line. The kinase SMG1 is one of the key components of the NMD system in many organisms, including plants. Several lines with a deleted SMG1 in a land Physcomitrium patens subsp. patens (“Gransden 2004”, Frieburg) were produced by James P. B. Lloyd [doi.org/10.1111/tpj.12329]. One of these lines, SMG1 KO mutant line 2, was used for this study. Protonemata of WT and mutant lines were grown in200 ml liquid BCD medium supplemented with 5 mM ammonium tartrate (BCDAT) during a 16-h photoperiod at 250C for 8 days [https://doi.org/10.1101/pdb.prot5136]. All experiments were performed in three biological replicates.
HostingRepositoryPRIDE
AnnounceDate2021-11-02
AnnouncementXMLSubmission_2021-11-02_06:47:45.334.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIgor Fesenko
SpeciesList scientific name: Physcomitrium patens; NCBI TaxID: 3218;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-18 05:20:06ID requested
12021-11-02 06:47:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Physcomitrium patens, iTRAQ, NMD
Contact List
Igor Fesenko
contact affiliationIBCH RAS
contact emailfesigor@gmail.com
lab head
Igor Fesenko
contact affiliationIBCH RAS
contact emailfesigor@gmail.com
dataset submitter
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Dataset FTP location
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