PXD029187

PXD029187 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomic analysis reveals the physiological regulatory networks of embryo dehydration protection in lotus (Nelumbo nucifera)
Description	Lotus is an aquatic plant that is sensitive to water loss, but its seeds are longevous after seed embryo dehydration and maturation. The great difference between the responses of vegetative organs and seeds to dehydration is related to the special protective mechanism in embryos. In this study, tandem mass tags (TMT)-labeled proteomics and parallel reaction monitoring (PRM) technologies were used to obtain novel insights into the physiological regulatory networks during lotus seed dehydration process. Totally, 60,266 secondary spectra and 32,093 unique peptides were detected. A total of 5,477 reliable proteins and 815 differentially expressed proteins (DEPs) were identified based on TMT data; of these, 582 DEPs were continuously down-regulated and 228 proteins were significantly up-regulated during the whole dehydration process. Bioinformatics and protein-protein interaction network analyses indicated that carbohydrate metabolism (including glycolysis/gluconeogenesis, galactose, starch and sucrose metabolism, pentose phosphate pathway, and cell wall organization), protein processing in ER, DNA repair, and antioxidative events had positive responses to lotus embryo dehydration. On the contrary, energy metabolism (metabolic pathway, photosynthesis, pyruvate metabolism, fatty acid biosynthesis) and secondary metabolism (terpenoid backbone, steroid, flavonoid biosynthesis) gradually become static status during lotus embryo water loss and maturation. Furthermore, non-enzymatic antioxidants and pentose phosphate pathway play major roles in antioxidant protection during dehydration process in lotus embryo; ABA signaling and the accumulation of oligosaccharides, late embryogenesis abundant proteins, and heat shock proteins may be the key factors to ensure the continuous dehydration and storage tolerance of lotus seed embryo. Stress physiology detection showed that H2O2 was the main ROS component inducing oxidative stress damage, and glutathione and vitamin E acted as the major antioxidant to maintain the REDOX balance of lotus embryo during the dehydration process. These results provide new insights to reveal the physiological regulatory networks of the protective mechanism of embryo dehydration in lotus.
HostingRepository	PRIDE
AnnounceDate	2021-12-19
AnnouncementXML	Submission_2021-12-19_02:07:38.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiangyuan Sheng
SpeciesList	scientific name: Nelumbo nucifera; NCBI TaxID: 4432;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-18 01:55:20	ID requested
⏵ 1	2021-12-19 02:07:39	announced

Dataset with its publication pending

submitter keyword: lotus, seed embryo, TMT proteomics

Di Zhang
contact affiliation	School of Design, Shanghai Jiao Tong University, Shanghai 200240, China
contact email	zhangdi2013@sjtu.edu.cn
lab head
Jiangyuan Sheng
contact affiliation	Shanghai Jiao Tong University
contact email	1033261039@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029187
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomic analysis reveals the physiological regulatory networks of embryo dehydration protection in lotus (Nelumbo nucifera)