PXD029169

PXD029169 is an original dataset announced via ProteomeXchange.

Title	Reversible flower opening regulated by CPK16-dependent phosphorylation and activation of aquaporin PIP2;2 in Gentiana
Description	Flower opening is important for successful pollination in many plant species, and some species repeat reversible flower opening and closing movements. This is thought to be due to the turgor pressure change caused by the water influx/efflux, which depends on osmotic oscillation in the cells. In some ornamental plants, it has been suggested that water channel aquaporin may play an important role in flower opening. However, the molecular mechanism(s) involved in flower movement are largely unknown. Using Gentiana flowers, which show reversible movement in response to temperature and light stimuli, as a model, we showed that reversible flower opening is regulated by aquaporin GsPIP2;2 and GsPIP2;7. In particular, phosphorylation of the C-terminal serine residue of GsPIP2;2 is important for the transport activity and correlates closely with the flower re-opening rate. Furthermore, GsPIP2;2 is phosphorylated and activated by GsCPK16, which is activated by elevated cytosolic Ca2+ levels in response to temperature and light stimuli. We propose that reversible flower opening is regulated by GsCPK16-dependent GsPIP2;2 phosphorylation and activation, with stimulus-induced calcium signals acting as triggers. CPK-dependent phosphorylation and activation of PIP2s may be one of the universal regulatory mechanisms for flower opening in plants.
HostingRepository	jPOST
AnnounceDate	2022-10-17
AnnouncementXML	Submission_2022-10-16_08:00:04.212.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomoya Niinae
SpeciesList	scientific name: Gentiana; NCBI TaxID: 21496;
ModificationList	S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide; unknown modification; unknown modification
Instrument	instrument

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-17 02:42:39	ID requested
⏵ 1	2022-10-16 08:00:04	announced

Dataset with its publication pending

submitter keyword: Phosphoproteome, TMT

Keiichirou Nemoto
lab head
Tomoya Niinae
contact affiliation	Kyoto university
dataset submitter

jPOST dataset URI

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