PXD029144 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics and immunoproteomics of Anoplocephala perfoliata |
Description | Anoplocephala perfoliata is a common tapeworm in horse with an ability to cause colic and increase mortality. At present, current diagnostic methods are not sensitive enough to detect A. perfoliata and immunoreactive excretory/secretory (E/S) proteins produced by this parasite, which comprise the most promising candidates for diagnostic tests. Here, we compared the E/S proteins produced by A. perfoliata worms of two different physiological states (small- and large-sized worms) in vitro by label-free quantitative (LFQ) proteomics with protein database composed of proteins encoded by Hymenolepis diminuta, Echinococcus multilocularis/granulosus and Taenia aseatica for protein identifications. The quantitative proteomics data was complemented with one-dimensional gel electrophoresis combined with immunoblotting using antisera from horses diagnosed positive and negative for this tapeworm. Protein bands cross-reacting most efficiently with the anti-A. perfoliata antibodies were subjected to in-gel digestion with LC-MS/MS identification. Both the non-gel and gel-based proteomic results were compared to propose a list of candidate proteins for further studies. These proteins will be validated for their suitability as diagnostic markers specific to cestodes and A. perfoliata. |
HostingRepository | PRIDE |
AnnounceDate | 2022-11-11 |
AnnouncementXML | Submission_2022-11-11_00:32:26.038.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | TuulaNyman |
SpeciesList | scientific name: Anoplocephala perfoliata; NCBI TaxID: 218192; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-15 03:22:05 | ID requested | |
⏵ 1 | 2022-11-11 00:32:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Perseus, E/S proteins, Tapeworm,Parasite, Horse, MaxQuant, LC-MS/MS, 1D immunoblotting |
Contact List
TuulaNyman |
contact affiliation | Head of Proteomics |
contact email | tuula.nyman@medisin.uio.no |
lab head | |
TuulaNyman |
contact affiliation | University of Oslo |
contact email | tuula.nyman@medisin.uio.no |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029144
- Label: PRIDE project
- Name: Proteomics and immunoproteomics of Anoplocephala perfoliata