PXD029120 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | G3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate cancer |
Description | SPOP, an E3 ubiquitin ligase, acts as a prostate-specific tumor suppressor with several key substrates mediating oncogenic function. However, the mechanisms underlying SPOP regulation are largely unknown. Here, we have identified G3BP1 as an interactor of SPOP and functions as a competitive inhibitor of Cul3SPOP, suggesting a distinctive mode of Cul3SPOP inactivation in prostate cancer (PCa). Transcriptomic analysis and functional studies reveal a G3BP1-SPOP ubiquitin signaling axis that promotes PCa progression through activating AR signaling. Moreover, AR directly upregulates G3BP1 transcription to further amplify G3BP1-SPOP signaling in a feed-forward manner. Our study supports a fundamental role of G3BP1 in disabling the tumor suppressive Cul3SPOP, thus defining a PCa cohort independent of SPOP mutation. Therefore, there are significantly more PCa that are defective for SPOP ubiquitin ligase than previously appreciated, and these G3BP1high PCa are more susceptible to AR-targeted therapy. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-25 |
AnnouncementXML | Submission_2021-11-25_06:59:18.624.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chandrani Mukhopadhyay |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-13 23:04:06 | ID requested | |
⏵ 1 | 2021-11-25 06:59:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Human Proteome Project |
submitter keyword: TAP-MS, Human, Prostate Cancer, SPOP, G3BP1 |
Contact List
Pengbo Zhou |
contact affiliation | Department of Pathology and Laboratory Medicine, The Joan and Stanford I. Weill Medical College of Cornell University, New York, NY 10065, USA |
contact email | pez2001@med.cornell.edu |
lab head | |
Chandrani Mukhopadhyay |
contact affiliation | Weill Cornell Medicine |
contact email | chm2067@med.cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD029120
- Label: PRIDE project
- Name: G3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate cancer