PXD028963 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain |
Description | In order to combat molecular damage, most cellular proteins undergo rapid turnover. We have previously identified large nuclear protein assemblies that can persist for years in post-mitotic tissues and are subject to age-related decline. Here we report that mitochondria can be long-lived in the mouse brain and reveal that specific mitochondrial proteins have half-lives longer than the average proteome. These mitochondrial long-lived proteins (mitoLLPs) are core components of the electron transport chain (ETC) and display increased longevity in respiratory supercomplexes. We find that COX7C, a mitoLLP that forms a stable contact site between Complexes I and IV, is required for Complex IV and supercomplex assembly. Remarkably, even upon depletion of COX7C transcripts, ETC function is maintained for days, effectively uncoupling mitochondrial function from ongoing transcription of its mitoLLPs. Our results suggest that modulating protein longevity within the ETC is critical for mitochondrial proteome maintenance and the robustness of mitochondrial function. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-04 |
AnnouncementXML | Submission_2021-11-04_05:07:05.960.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028963 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Shefali Krishna |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | 6x(13)C labeled residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-06 06:05:08 | ID requested | |
⏵ 1 | 2021-11-04 05:07:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mitochondria |
protein homeostasis |
long-lived proteins |
electron transport chain |
Contact List
Martin Hetzer |
contact affiliation | Salk Institute, La Jolla, CA USA |
contact email | hetzer@salk.edu |
lab head | |
Shefali Krishna |
contact affiliation | Salk Institute of Biological Studies |
contact email | skrishna@salk.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028963
- Label: PRIDE project
- Name: Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain