PXD028945

PXD028945 is an original dataset announced via ProteomeXchange.

Title	GFI1 tethers the NuRD complex to open and transcriptionally active chromatin in myeloid progenitors
Description	GFI is a DNA binding transcriptional repressor that regulates myeloid differentiation. Here, we show that GFI1 interacts with the chromodomain helicase CHD4 and other components of the nucleosome remodeling and deacetylase (NuRD) complex. Our data demonstrated that GFI1 and GFI1/CHD4 complexes occupy sites of open chromatin enriched for histone marks associated with active transcription or different sets of genes that are either enriched for IRF1 or SPI-1 consensus binding sites. In addition, our study provided evidence that GFI1 affects the chromatin remodeling activity of the NuRD complex. Overall, our results indicate that GFI1/CHD4 complexes control chromatin openness and histone modifications differentially to regulate target genes, which govern the immune response, nucleosome organization, or metabolic processes.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_06:58:34.210.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD028945
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Halil Bagci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-05 02:00:24	ID requested
⏵ 1	2021-11-02 06:58:34	announced

Dataset with its publication pending

submitter keyword: GFI1, NuRD complex, chromatin, myeloid differentiation

Tarik Moroy
contact affiliation	Institut de recherches cliniques de Montreal, Montreal, QC, H2W 1R7, Canada
contact email	Tarik.Moroy@ircm.qc.ca
lab head
Halil Bagci
contact affiliation	ETH Zurich
contact email	halil.bagci@mail.mcgill.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028945
     1. Label: PRIDE project
     2. Name: GFI1 tethers the NuRD complex to open and transcriptionally active chromatin in myeloid progenitors