PXD028853

PXD028853 is an original dataset announced via ProteomeXchange.

Title	Enhancing Cysteine Chemoproteomic Coverage Through Systematic Assessment of Click Chemistry Product Fragmentation
Description	Higher-energy C-trap dissociation (HCD) of triazole-and biotin-modified peptides affords several characteristic fragment ions, including previously reported and newly identified species. Using the open and mass offset search strategies, we conduct an in-depth analysis of the relative intensities and specificity of these signature fragments. Through comparison of structurally matched isotopologues, we pinpoint the nature of the observed fragments.By varying the nature of the labeling reagent, including linker length, number of fragmentable bonds, and position of the triazole, we also achieve improved coverage of labeled peptides. The combination of labile and non-labile ion searches also affords an improvement in the chemoproteomic detected (CpD) cysteines identified. Collectively our study demonstrates the utility of labile ion and mass offset search strategies in the analysis of chemoproteomics datasets and reveals the ubiquity of triazole fragmentation in MS/MS analysis of chemically modified peptides.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:34:23.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianyang Yan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-29 14:01:11	ID requested
⏵ 1	2021-11-03 05:34:24	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, Fragmentation, Cysteines, Chemoproteomics

Keriann M. Backus
contact affiliation	Biological Chemistry Department, David Geffen School of Medicine, UCLA
contact email	KBackus@mednet.ucla.edu
lab head
Tianyang Yan
contact affiliation	Department of Chemistry and Biochemistry, UCLA
contact email	tyyan14@outlook.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028853
     1. Label: PRIDE project
     2. Name: Enhancing Cysteine Chemoproteomic Coverage Through Systematic Assessment of Click Chemistry Product Fragmentation