PXD028727
PXD028727 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Deciphering the molecular mechanism of long non-coding RNA HIFA-AS1 regulating pancreatic cancer cells |
| Description | HIFA-AS1, an antisense transcript of HIF1α gene, is a 652-bp long non-coding RNA (lncRNA) which globally expressed in multiple tissues of animals. Recent evidence indicated that the HIFA-AS1 was involved in tumorigenesis of several types of cancer, but there were no reports on pancreatic cancer (PC). In order to investigate whether the HIFA-AS1 could mediate the PC or not, it was overexpressed in a PC cell line (PANC-1), and a series of experiments including cell viability detection, flow cytometry, and transwell migration were performed. Functionally, the results indicated that overexpression (OE) of HIFA-AS1 could inhibit proliferation and shift, and promote apoptosis of PC cells. Moreover, to explore underlying molecular mechanism of HIFA-AS1 in PC cells, the iTRAQ (isobaric tags for relative and absolute quantification) quantitative proteomics analysis was implemented. Mechanistically, it was found that OE of HIFA-AS1 globally affected the expression levels of multiple protein associated with metabolism of cancer, suggesting that HIFA-AS1 might regulate the tumorigenesis of PC by up- and down-regulating the gene expression. |
| HostingRepository | iProX |
| AnnounceDate | 2021-09-23 |
| AnnouncementXML | Submission_2021-09-23_19:29:30.887.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | He Zhang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-09-23 19:28:47 | ID requested | |
| ⏵ 1 | 2021-09-23 19:29:31 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: HIFA-AS1, iTRAQ |
Contact List
| Jing Tian | |
|---|---|
| contact affiliation | General Hospital of Northern Theater Command, China |
| contact email | 13352459336@163.com |
| lab head | |
| He Zhang | |
| contact affiliation | General Hospital of Northern Theater Command, China |
| contact email | hzauzhoujian@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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