PXD028713 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic and phosphoproteomic of C2C12 myogenesis and MAPK inhibition |
Description | Myogenesis is governed by signalling networks whose regulations are tightly controlled in a time-dependent manner. While different protein kinases have been identified to regulate various aspects of myogenesis, knowledge on the global signalling networks and their downstream substrates during myogenesis remains incomplete. Here, we map the myogenic differentiation of C2C12 cells using mass spectrometry (MS)-based phosphoproteomics and proteomics. From these data, we infer global kinase activity and predict substrates of key kinases that are involved in myogenesis. We found that multiple mitogen-activated protein kinases (MAPKs) mark the initial wave of signalling cascades. Further phosphoproteomic and proteomic profiling with MAPK1/3 and MAPK8/9 specific inhibitions unveil their shared and distinctive roles on myogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-31 |
AnnouncementXML | Submission_2022-05-31_03:04:27.816.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Benjamin Parker |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-22 23:36:18 | ID requested | |
⏵ 1 | 2022-05-31 03:04:28 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: C2C12, myogenesis, MAPK |
Contact List
Benjamin Parker |
contact affiliation | The University of Melbourne |
contact email | ben.parker@unimelb.edu.au |
lab head | |
Benjamin Parker |
contact affiliation | The University of Melbourne |
contact email | ben.parker@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028713
- Label: PRIDE project
- Name: Proteomic and phosphoproteomic of C2C12 myogenesis and MAPK inhibition