PXD028704 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry |
Description | Cell cycle progression into mitosis induce cellular rearrangements such as mitotic spindle formation, Golgi fragmentation, and nuclear envelope breakdown. Like certain retroviruses, nuclear delivery of HPV16 genomes is facilitated by these processes during entry into host cells by tethering of the viral DNA to mitotic chromosomes through the minor capsid protein L2. However, the mechanism of delivery onto and tethering to the condensed chromosomes is barely understood on a mechanistic level. To date it is unclear, which cellular proteins facilitate this process in interaction with L2 or how this process is regulated. Here, we discovered that HPV16 minor capsid protein L2 is phosphorylated during entry upon mitosis onset on conserved residues within the chromosome-binding region (CBR) that is responsible for nuclear import. The crucial L2 phosphorylations occurred sequentially by the master mitotic kinases CDK1 and PLK1. L2 phosphorylation, thus, not only regulated timely delivery of HPV16 vDNA to mitotic chromatin at mitosis onset, but also likely resulted in a conformational switch in L2 that allowed engagement of cellular proteins for this purpose. In summary, our work demonstrates for the first time a crucial role of mitotic kinases for nuclear entry of a DNA virus and provides important insights into the molecular mechanism of pathogen import into the nucleus during mitosis. |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-22 |
AnnouncementXML | Submission_2022-12-22_03:19:01.084.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | HannesDrexler |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human papillomavirus type 16; NCBI TaxID: 333760; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-22 12:10:29 | ID requested | |
⏵ 1 | 2022-12-22 03:19:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MSMS, HPV, proteomics, CDK1, mitosis, human papillomavirus, PLK1,Virus entry, nuclear import |
Contact List
Hannes C. A.Drexler |
contact affiliation | Max Planck Institute for Moleclar Biomedicine Bioanalyticyl Mass Spectrometry Unit Röntgenstr. 20 48149 Münster Germany |
contact email | hannes.drexler@mpi-muenster.mpg.de |
lab head | |
HannesDrexler |
contact affiliation | Bioanalytical Mass Spectrometry |
contact email | hannes.drexler@mpi-muenster.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028704
- Label: PRIDE project
- Name: Master mitotic kinases regulate viral genome delivery during papillomavirus cell entry