PXD028666

PXD028666 is an original dataset announced via ProteomeXchange.

Title	Elucidation of the translation initiation factor interaction network of Haloferax volcanii reveals coupling of transcription and translation in haloarchaea
Description	Translation is an important step in gene expression. Initiation of translation is rate-limiting, and it is 13 phylogenetically more diverse than elongation or termination. Bacteria contain only three initiation 14 factors. In stark contrast, eukaryotes contain more than 10 (subunits of) initiation factors (eIFs). The 15 genomes of archaea contain many genes that are annotated to encode archaeal homologs of 16 eukaryotic initiation factors (aIFs). However, experimental characterization of aIFs is scarce and 17 mostly restricted to very few species. To broaden the view, the protein-protein interaction network of 18 aIFs in the halophilic archaeon Haloferax volcanii has been characterized. To this end, tagged 19 versions of 14 aIFs were overproduced, affinity isolated, and the co-isolated binding partners were 20 identified by peptide mass fingerprinting.
HostingRepository	PRIDE
AnnounceDate	2021-09-29
AnnouncementXML	Submission_2021-09-29_03:57:14.812.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uwe Linne
SpeciesList	scientific name: Haloferax volcanii; NCBI TaxID: 2246;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-20 23:59:42	ID requested
⏵ 1	2021-09-29 03:57:15	announced

Dataset with its publication pending

submitter keyword: Haloferax volcanii, translation initiation, aIF, ribosome, RNA polymerase, interaction networks, transcription, coupling

Dr. Uwe Linne
contact affiliation	Dr. Uwe Linne Philipps-Universität Marburg Fachbereich Chemie und Synmikro Gerätezentrum Massenspektrometrie und Elementanalaytik Hans-Meerwein-Str. 4 35032 Marburg, GERMANY
contact email	linneu@staff.uni-marburg.de
lab head
Uwe Linne
contact affiliation	Head of Core Facility Mass Spectrometry, Faculty of Chemistry, Philipps-University Marburg
contact email	linneu@staff.uni-marburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD028666

PRIDE project URI

• PRIDE
  1. PXD028666
     1. Label: PRIDE project
     2. Name: Elucidation of the translation initiation factor interaction network of Haloferax volcanii reveals coupling of transcription and translation in haloarchaea