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PXD028594

PXD028594 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRadiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment
DescriptionObjectives: Salivary glands are affected during radiotherapy in the head and neck region, leading to a reduction in salivary flow and changes its composition. Besides negatively affecting the oral soft tissues, this can also lead to dental impairment. Thus, we evaluated the effect of radiotherapy in the proteomic profile of the saliva in patients with head-and-neck-cancer (HNC). Materials and methods: HNC patients had their saliva collected before (BRT), during (2-5 weeks; DRT) and after (3-4 months; ART) radiotherapy. Saliva was also collected from healthy volunteers (control; C). Samples were processed for proteomic analysis. Results: In total 1.055 proteins were identified, among which 46 were common to all groups, while 86, 86, 286 and 395 were exclusively found in C, BRT, DRT and ART, respectively. Remarkably, alpha-enolase was increased 35-fold DRT compared with BRT, while proline-rich proteins were decreased. ART there was a 16-fold increase in scaffold attachment factor-B1 and a 3-fold decrease in alpha-enolase and several cystatins. When compared with C, salivary proteins of BRT patients showed increases cystatin-C, lysozyme C, histatin-1 and proline-rich proteins. Conclusion/Clinical revelance: Both HNC and radiotherapy remarkably change the salivary protein composition. Altogether, our results, for the first time, suggest investigating alpha-enolase levels in saliva DRT in future studies as a possible biomarker and strategy to predict the efficiency of the treatment. Moreover, our data provide important insights for designing dental products that are more effective for these patients and contribute to a better understanding of the progressive changes in salivary proteins induced by radiotherapy.
HostingRepositoryPRIDE
AnnounceDate2021-10-12
AnnouncementXMLSubmission_2021-10-12_05:51:33.638.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTalita Ventura
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentXevo G2 Q-Tof; nanoACQUITY UPLC
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 22:56:55ID requested
12021-10-12 05:51:33announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: saliva, proteomics, head and neck cancer, radiotherapy, biomarkes, dental caries
Contact List
Talita Mendes Oliveira Ventura
contact affiliationDepartment of Biological Science - Laboratory of Biochemistry. Bauru School of Dentistry - University of São Paulo.
contact emailtalitaventura@usp.br
lab head
Talita Ventura
contact affiliationUniversity of São Paulo, Bauru Dental School
contact emailtalitaventura@usp.br
dataset submitter
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