PXD028594 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment |
Description | Objectives: Salivary glands are affected during radiotherapy in the head and neck region, leading to a reduction in salivary flow and changes its composition. Besides negatively affecting the oral soft tissues, this can also lead to dental impairment. Thus, we evaluated the effect of radiotherapy in the proteomic profile of the saliva in patients with head-and-neck-cancer (HNC). Materials and methods: HNC patients had their saliva collected before (BRT), during (2-5 weeks; DRT) and after (3-4 months; ART) radiotherapy. Saliva was also collected from healthy volunteers (control; C). Samples were processed for proteomic analysis. Results: In total 1.055 proteins were identified, among which 46 were common to all groups, while 86, 86, 286 and 395 were exclusively found in C, BRT, DRT and ART, respectively. Remarkably, alpha-enolase was increased 35-fold DRT compared with BRT, while proline-rich proteins were decreased. ART there was a 16-fold increase in scaffold attachment factor-B1 and a 3-fold decrease in alpha-enolase and several cystatins. When compared with C, salivary proteins of BRT patients showed increases cystatin-C, lysozyme C, histatin-1 and proline-rich proteins. Conclusion/Clinical revelance: Both HNC and radiotherapy remarkably change the salivary protein composition. Altogether, our results, for the first time, suggest investigating alpha-enolase levels in saliva DRT in future studies as a possible biomarker and strategy to predict the efficiency of the treatment. Moreover, our data provide important insights for designing dental products that are more effective for these patients and contribute to a better understanding of the progressive changes in salivary proteins induced by radiotherapy. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-12 |
AnnouncementXML | Submission_2021-10-12_05:51:33.638.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Talita Ventura |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Xevo G2 Q-Tof; nanoACQUITY UPLC |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-16 22:56:55 | ID requested | |
⏵ 1 | 2021-10-12 05:51:33 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: saliva, proteomics, head and neck cancer, radiotherapy, biomarkes, dental caries |
Contact List
Talita Mendes Oliveira Ventura |
contact affiliation | Department of Biological Science - Laboratory of Biochemistry. Bauru School of Dentistry - University of São Paulo. |
contact email | talitaventura@usp.br |
lab head | |
Talita Ventura |
contact affiliation | University of São Paulo, Bauru Dental School |
contact email | talitaventura@usp.br |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028594
- Label: PRIDE project
- Name: Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during and after treatment