PXD028543

PXD028543 is an original dataset announced via ProteomeXchange.

Title	Chlamydia trachomatis effector Dre1 interacts with dynactin to reposition host organelles during infection.
Description	Chlamydia trachomatis is an obligate intracellular pathogen that replicates within a specialized membrane-bound compartment, the inclusion. Chlamydia species express a unique class of effectors, Incs, which are translocated from the bacteria by a Type III secretion system and are inserted into the inclusion membrane where they modulate the host-bacterium interface. C. trachomatis repositions specific host organelles during infection to acquire nutrients and evade host cell surveillance, however the bacterial and host proteins controlling these processes are largely unknown. Here, we identify an interaction between the host dynactin complex and the C. trachomatis Inc CT192 (CTL0444), hereafter named Dre1 for Dynactin Recruiting Effector 1. We show that dynactin is recruited to the inclusion in a Dre1-dependent manner and that inactivation of Dre1 diminishes the recruitment of specific host organelles, including the centrosome, mitotic spindle, and Golgi Apparatus to the inclusion. Inactivation of Dre1 results in decreased C. trachomatis fitness in cell-based assays and in a mouse model of infection. By targeting particular functions of the versatile host dynactin complex, Dre1 facilitates re-arrangement of certain organelles around the growing inclusion. Our work highlights how C. trachomatis employs a single effector to evoke specific, large-scale changes in host cell organization that establish the intracellular replicative niche without globally inhibiting host cellular function.
HostingRepository	PRIDE
AnnounceDate	2025-03-31
AnnouncementXML	Submission_2025-03-31_07:59:43.651.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danielle Swaney
SpeciesList	scientific name: Chlamydia trachomatis; NCBI TaxID: 813; scientific name: Chlamydia trachomatis; NCBI TaxID: NCBITaxon:813; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 06:43:26	ID requested
⏵ 1	2025-03-31 07:59:44	announced

Dataset with its publication pending

submitter keyword: Chlamydia, Human, protein-protein interaction

Nevan Krogan
contact affiliation	University of California San Francisco
contact email	daniswan@gmail.com
lab head
Danielle Swaney
contact affiliation	UCSF
contact email	daniswan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028543
     1. Label: PRIDE project
     2. Name: Chlamydia trachomatis effector Dre1 interacts with dynactin to reposition host organelles during infection.