PXD028530 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism |
Description | NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the auxin-dependent plant phototropic growth response. We report that NPH3 directly binds polyacidic phospholipids, required for plasma membrane association in darkness. We further demonstrate that blue light induces an immediate phosphorylation of a C-terminal 14-3-3 binding motif in NPH3. Subsequent association of 14-3-3 proteins is causal for the light-induced release of NPH3 from the membrane and accompanied by NPH3 dephosphorylation. In the cytosol, NPH3 dynamically transitions into membrane-less condensate-like structures. The dephosphorylated state of the 14-3-3 binding site and NPH3 membrane recruitment are recoverable in darkness. NPH3 variants that constitutively localize either to the membrane or to condensates are non-functional, revealing a fundamental role of the 14-3-3 mediated dynamic change in NPH3 localization for auxin-dependent phototropism. This regulatory mechanism might be of general nature, given that several members of the NPH3-like family interact with 14-3-3 via a C-terminal motif. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-12 |
AnnouncementXML | Submission_2021-10-12_05:27:12.501.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nicolas Nalpas |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-16 06:41:48 | ID requested | |
⏵ 1 | 2021-10-12 05:27:12 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Arabidopsis thaliana, etiolated seedling, blue-light dependent 14-3-3 interactors |
Contact List
Claudia Oecking |
contact affiliation | Center for Plant Molecular Biology (ZMBP) Plant Physiology University of Tübingen Tübingen Germany |
contact email | claudia.oecking@zmbp.uni-tuebingen.de |
lab head | |
Nicolas Nalpas |
contact affiliation | Tuebingen University |
contact email | nalpas.nicolas@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD028530 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028530
- Label: PRIDE project
- Name: Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism