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PXD028530

PXD028530 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLight-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism
DescriptionNON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the auxin-dependent plant phototropic growth response. We report that NPH3 directly binds polyacidic phospholipids, required for plasma membrane association in darkness. We further demonstrate that blue light induces an immediate phosphorylation of a C-terminal 14-3-3 binding motif in NPH3. Subsequent association of 14-3-3 proteins is causal for the light-induced release of NPH3 from the membrane and accompanied by NPH3 dephosphorylation. In the cytosol, NPH3 dynamically transitions into membrane-less condensate-like structures. The dephosphorylated state of the 14-3-3 binding site and NPH3 membrane recruitment are recoverable in darkness. NPH3 variants that constitutively localize either to the membrane or to condensates are non-functional, revealing a fundamental role of the 14-3-3 mediated dynamic change in NPH3 localization for auxin-dependent phototropism. This regulatory mechanism might be of general nature, given that several members of the NPH3-like family interact with 14-3-3 via a C-terminal motif.
HostingRepositoryPRIDE
AnnounceDate2021-10-12
AnnouncementXMLSubmission_2021-10-12_05:27:12.501.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNicolas Nalpas
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 06:41:48ID requested
12021-10-12 05:27:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis thaliana, etiolated seedling, blue-light dependent 14-3-3 interactors
Contact List
Claudia Oecking
contact affiliationCenter for Plant Molecular Biology (ZMBP) Plant Physiology University of Tübingen Tübingen Germany
contact emailclaudia.oecking@zmbp.uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliationTuebingen University
contact emailnalpas.nicolas@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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