PXD028524

PXD028524 is an original dataset announced via ProteomeXchange.

Title	Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection
Description	Reproductive traits that influence female remating and competitive fertilization rapidly evolve in response to sexual selection and sexual conflict. One such trait, observed across diverse animal taxa, is the formation of a structural plug inside the female reproductive tract, either during or shortly after mating. In Drosophila melanogaster, male seminal fluid forms a mating plug inside the female bursa, which has been demonstrated to influence sperm entry into storage and latency of female remating. Processing of the plug, including its eventual ejection from the female's reproductive tract, influences the competitive fertilization success of her mates and is mediated by female × male genotypic interactions. However, female contributions to plug formation and processing have received limited attention. Using developmental mutants that lack glandular female reproductive tract tissues, we reveal that these glandular tissues are essential for the mating plug to be ejected. We further use proteomics to demonstrate that female glandular proteins, and especially proteolytic enzymes, contribute to mating plug composition and that the absence of glands has a widespread impact of plug formation and composition. Together, these phenotypic and molecular data resolve molecular mechanisms of important postmating, intersexual interactions and cryptic female choice.
HostingRepository	PRIDE
AnnounceDate	2022-01-25
AnnouncementXML	Submission_2022-01-25_03:29:06.066.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Caitlin McDonough-Goldstein
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 06:41:17	ID requested
⏵ 1	2022-01-25 03:29:06	announced

Dataset with its publication pending

submitter keyword: spermatheca, parovaria, mating plug, sperm ejection, ejaculate-female interactions, female reproductive tract

Steve Dorus
contact affiliation	Center for Reproductive Evolution, Biology Department, Syracuse University
contact email	sdorus@syr.edu
lab head
Caitlin McDonough-Goldstein
contact affiliation	Syracuse University
contact email	mcdonouce@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD028524

PRIDE project URI

• PRIDE
  1. PXD028524
     1. Label: PRIDE project
     2. Name: Drosophila female reproductive glands contribute to mating plug composition and the timing of sperm ejection