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PXD028502

PXD028502 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCorrelative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and macrophages
DescriptionOpportunistic pathogen Acinetobacter baumannii possesses stress tolerance strategies against host innate immunity and antibiotic killing. However, how the host-pathogen-antibiotic interaction affects the overall molecular regulation of pro- and anti-pathogenic machineries remains unexplored. Here, we simultaneously investigate proteomic changes in A. baumannii and macrophages following infection in the absence or presence of the last-line polymyxins. We discover that macrophages and polymyxins exhibit complementary effects to disarm several A. baumannii stress tolerance and survival strategies, including oxidative stress resistance, copper tolerance, bacterial iron acquisition and stringent response regulation systems. Using bacterial mutants with impaired stringent response associated (p)ppGpp synthetase/hydrolase spoT we demonstrate that spot mutants exhibit significantly enhanced susceptibility to polymyxin killing and reduced in vivo survivability compared to the wild-type strain. Together, our findings highlight that improved understanding of host-pathogen-antibiotic interplay is critical for optimisation of antibiotic use in patients and discovery of new antibiotics to tackle multidrug-resistant bacterial infections.
HostingRepositoryPRIDE
AnnounceDate2022-02-11
AnnouncementXMLSubmission_2022-02-11_07:01:30.698.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRalf Schittenhelm
SpeciesList scientific name: Acinetobacter baumannii; NCBI TaxID: 470; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-16 02:12:09ID requested
12022-02-11 07:01:31announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Acinetobacter baumannii, polymyxin, macrophage, proteomic, stringent response
Contact List
Jian Li
contact affiliationBiomedicine Discovery Institute, Infection Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia
contact emailjian.li@monash.edu
lab head
Ralf Schittenhelm
contact affiliationMonash University
contact emailralf.schittenhelm@monash.edu
dataset submitter
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Dataset FTP location
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