PXD028502 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and macrophages |
Description | Opportunistic pathogen Acinetobacter baumannii possesses stress tolerance strategies against host innate immunity and antibiotic killing. However, how the host-pathogen-antibiotic interaction affects the overall molecular regulation of pro- and anti-pathogenic machineries remains unexplored. Here, we simultaneously investigate proteomic changes in A. baumannii and macrophages following infection in the absence or presence of the last-line polymyxins. We discover that macrophages and polymyxins exhibit complementary effects to disarm several A. baumannii stress tolerance and survival strategies, including oxidative stress resistance, copper tolerance, bacterial iron acquisition and stringent response regulation systems. Using bacterial mutants with impaired stringent response associated (p)ppGpp synthetase/hydrolase spoT we demonstrate that spot mutants exhibit significantly enhanced susceptibility to polymyxin killing and reduced in vivo survivability compared to the wild-type strain. Together, our findings highlight that improved understanding of host-pathogen-antibiotic interplay is critical for optimisation of antibiotic use in patients and discovery of new antibiotics to tackle multidrug-resistant bacterial infections. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-11 |
AnnouncementXML | Submission_2022-02-11_07:01:30.698.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ralf Schittenhelm |
SpeciesList | scientific name: Acinetobacter baumannii; NCBI TaxID: 470; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-16 02:12:09 | ID requested | |
⏵ 1 | 2022-02-11 07:01:31 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Acinetobacter baumannii, polymyxin, macrophage, proteomic, stringent response |
Contact List
Jian Li |
contact affiliation | Biomedicine Discovery Institute, Infection Program, Department of Microbiology, Monash University, Clayton, Victoria, Australia |
contact email | jian.li@monash.edu |
lab head | |
Ralf Schittenhelm |
contact affiliation | Monash University |
contact email | ralf.schittenhelm@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028502
- Label: PRIDE project
- Name: Correlative proteomics identify the key roles of stress tolerance strategies in Acinetobacter baumannii in response to polymyxin and macrophages