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PXD028484

PXD028484 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDrosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins
DescriptionDescription Drosophila melanogaster nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that present a target for insecticides. However, a better understanding of in vioreceptor subunit composition is needed for effective design of insecticides. Alpha-neurotxins are known as peptide toxins capable to block nAChRs by binding to its target subunits. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten nAChR subunit genes. We studied interactions of nAChRs to a snake toxin by using styrene maleic acid lipid particles (SMALPs) extraction method. For the null alleles which are impaired in locomotor activities we examined the binding of nAChRs incorporated into SMALPs to alpha bungarotoxin by employing mass spectrometry. We show native ligand interactions to Dalpha5, Dalpha6 and Dalpha7 subunits and reveal glycosylation sites of Dalpha5 and Dalpha7 by a glycoproteomic approach. Finally, we report the localization of fluorescent tagged Dalpha6 across nervous system development
HostingRepositoryPRIDE
AnnounceDate2021-10-12
AnnouncementXMLSubmission_2021-10-11_16:03:59.121.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD028484
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKathryn Lilley
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-15 14:26:46ID requested
12021-10-11 16:03:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: nAChRs, SMALPs, pull-down,toxins
Contact List
Kathryn S. Lilley
contact affiliationCambridge Centre for Proteomics Department of Biochemistry University of Cambridge
contact emailksl23@cam.ac.uk
lab head
Kathryn Lilley
contact affiliationUniversity of Cambridge
contact emailksl23@cam.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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