PXD028484 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins |
Description | Description Drosophila melanogaster nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that present a target for insecticides. However, a better understanding of in vioreceptor subunit composition is needed for effective design of insecticides. Alpha-neurotxins are known as peptide toxins capable to block nAChRs by binding to its target subunits. To facilitate the analysis of nAChRs we used a CRISPR/Cas9 strategy to generate null alleles for all ten nAChR subunit genes. We studied interactions of nAChRs to a snake toxin by using styrene maleic acid lipid particles (SMALPs) extraction method. For the null alleles which are impaired in locomotor activities we examined the binding of nAChRs incorporated into SMALPs to alpha bungarotoxin by employing mass spectrometry. We show native ligand interactions to Dalpha5, Dalpha6 and Dalpha7 subunits and reveal glycosylation sites of Dalpha5 and Dalpha7 by a glycoproteomic approach. Finally, we report the localization of fluorescent tagged Dalpha6 across nervous system development |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-12 |
AnnouncementXML | Submission_2021-10-11_16:03:59.121.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD028484 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Kathryn Lilley |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-15 14:26:46 | ID requested | |
⏵ 1 | 2021-10-11 16:03:59 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: nAChRs, SMALPs, pull-down,toxins |
Contact List
Kathryn S. Lilley |
contact affiliation | Cambridge Centre for Proteomics Department of Biochemistry University of Cambridge |
contact email | ksl23@cam.ac.uk |
lab head | |
Kathryn Lilley |
contact affiliation | University of Cambridge |
contact email | ksl23@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD028484
- Label: PRIDE project
- Name: Drosophila nicotinic acetylcholine receptor subunits and their native interactions with insecticidal peptide toxins