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PXD028396

PXD028396 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleKlebsiella pneumoniae x546,ATCC15380, TMT
DescriptionKlebsiella pneumoniae x546,ATCC15380, TMT,The samples were ground into a powder in liquid nitrogen. Then the powder was suspended in lysis buffer [1% sodium deoxycholate (SDS), 8M urea]. The mixture was allowed to settle at 4 鎺矯 for 30 min during which the sample were vortexed at every 5 min, and treated by ultrasound at 40 kHz and 40 W for 2 min. After centrifugation at 16000 rpm at 4鎺矯 for 30 min, the concentration of protein supernatant was determined by Bicinchoninic acid (BCA) method by BCA Protein Assay Kit (Pierce, Thermo, USA). Protein quantification was performed according to the kit protocol(Chen et al., 2021)
HostingRepositoryPRIDE
AnnounceDate2021-11-15
AnnouncementXMLSubmission_2021-11-15_00:59:38.576.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxin wang
SpeciesList scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; scientific name: Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940; NCBI TaxID: 1162296; scientific name: Klebsiella pneumoniae MGH 78578; NCBI TaxID: 1328388;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-09-09 08:35:30ID requested
12021-11-15 00:59:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Klebsiella pneumoniae x546,ATCC15380, TMT
Contact List
Wang Xin
contact affiliationTianjin university
contact email546568836@qq.com
lab head
xin wang
contact affiliationTian Jin university
contact email546568836@qq.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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