PXD028396 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Klebsiella pneumoniae x546,ATCC15380, TMT |
Description | Klebsiella pneumoniae x546,ATCC15380, TMT,The samples were ground into a powder in liquid nitrogen. Then the powder was suspended in lysis buffer [1% sodium deoxycholate (SDS), 8M urea]. The mixture was allowed to settle at 4 鎺矯 for 30 min during which the sample were vortexed at every 5 min, and treated by ultrasound at 40 kHz and 40 W for 2 min. After centrifugation at 16000 rpm at 4鎺矯 for 30 min, the concentration of protein supernatant was determined by Bicinchoninic acid (BCA) method by BCA Protein Assay Kit (Pierce, Thermo, USA). Protein quantification was performed according to the kit protocol(Chen et al., 2021) |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-15 |
AnnouncementXML | Submission_2021-11-15_00:59:38.576.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | xin wang |
SpeciesList | scientific name: Klebsiella pneumoniae; NCBI TaxID: 573; scientific name: Klebsiella pneumoniae subsp. pneumoniae DSM 30104 = JCM 1662 = NBRC 14940; NCBI TaxID: 1162296; scientific name: Klebsiella pneumoniae MGH 78578; NCBI TaxID: 1328388; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-09-09 08:35:30 | ID requested | |
⏵ 1 | 2021-11-15 00:59:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Klebsiella pneumoniae x546,ATCC15380, TMT |
Contact List
Wang Xin |
contact affiliation | Tianjin university |
contact email | 546568836@qq.com |
lab head | |
xin wang |
contact affiliation | Tian Jin university |
contact email | 546568836@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD028396 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD028396
- Label: PRIDE project
- Name: Klebsiella pneumoniae x546,ATCC15380, TMT