PXD028351

PXD028351 is an original dataset announced via ProteomeXchange.

Title	The Hsp90 cochaperone TTT promotes cotranslational maturation of PIKK kinases prior to complex assembly
Description	PIKKs are a family of kinases that control fundamental processes, including cell growth, DNA damage repair, and gene expression. Although their regulation and activities are well characterized, little is known about how PIKKs fold and assemble into active complexes. Previous work identified an Hsp90 cochaperone, the TTT complex, which specifically stabilizes PIKKs. Here we describe a mechanism by which TTT promotes their de novo maturation in fission yeast. We show that TTT recognizes newly synthesized PIKKs during translation. Although PIKKs form multimeric complexes, we found that they do not engage in cotranslational assembly with their partners. Rather, we accumulated evidence that TTT protects nascent PIKK polypeptides from misfolding and degradation and that PIKKs acquire their native state after translation is terminated. Thus, PIKK maturation and assembly are temporally segregated, suggesting that the biogenesis of large complexes requires both dedicated chaperones and cotranslational interactions between subunits.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_23:32:44.849.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:28:24	ID requested
⏵ 1	2021-09-09 23:32:45	announced

Dataset with its publication pending

submitter keyword: PIKK kinases, Multimeric complex, Chaperone, Translation

Dominique Helmlinger
contact affiliation	Regulation of gene expression, CRBM, France
contact email	dhelmlinger@crbm.cnrs.fr
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028351
     1. Label: PRIDE project
     2. Name: The Hsp90 cochaperone TTT promotes cotranslational maturation of PIKK kinases prior to complex assembly