PXD028324

PXD028324 is an original dataset announced via ProteomeXchange.

Title	Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair
Description	Cross-talk between distinct protein post-translational modifications is critical for an effective DNA damage response. Arginine methylation plays an important role in maintaining genome stability, however how this modification integrates with other enzymatic activities is largely unknown. Here, we identify the deubiquitylating enzyme USP11 as a previously uncharacterised PRMT1 substrate, and that methylation of USP11 promotes DNA end-resection and the repair of DNA double strand breaks by homologous recombination (HR). In addition, we show that PRMT1 is a ubiquitylated protein that it is targeted for deubiquitylation by USP11, and that USP11 regulates the ability of PRMT1 to bind to and methylate MRE11. Interestingly, USP11 methylation by PRMT1 is not required for other USP11 activities during HR, such as PALB2 deubiquitylation. Taken together, our findings reveal a specific role for USP11 during the early stages of DSB repair, which is mediated through its ability to regulate the activity of the PRMT1-MRE11 pathway.
HostingRepository	PRIDE
AnnounceDate	2021-09-28
AnnouncementXML	Submission_2021-09-28_07:48:53.653.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Skehel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-09 08:25:40	ID requested
⏵ 1	2021-09-28 07:48:54	announced

Dataset with its publication pending

submitter keyword: Metylation, lc-ms/ms, DNA repair, ubiquitination, PRMT1

Clare C Davies
contact affiliation	Institute of Cancer and Genomic Sciences, University of Birmingham, UK
contact email	c.c.davies@bham.ac.uk
lab head
Mark Skehel
contact affiliation	Francis Crick Institute
contact email	skehelm@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD028324
     1. Label: PRIDE project
     2. Name: Arginine methylation and ubiquitylation crosstalk controls DNA end-resection and homologous recombination repair