PXD028030

PXD028030 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Characterization of covalent bonding of 4-methylcatechol to beta-lactoglobulin and the effect on in vitro protein digestibility
Description	Scope: 4-Methylcatechol (4MC) is a polyphenol with origin in processed food. Protein-polyphenol adducts are formed upon covalent bonding between oxidized polyphenols and proteins in food. The present study investigated in vitro gastric and intestinal digestion of a milk protein, beta-lactoglobulin (beta-LG), covalently modified at its nucleophilic amino acids by 4-methylbenzoquinone (4MBQ), the oxidized form of 4MC. Present results characterize 4MBQ-induced covalent modifications on beta-LG and their effect on protein digestibility. Methods and results: The beta-LG-4MBQ adduct (beta-LQ) was prepared at pH 8 by reacting beta-LG and 4MBQ in equimolar ratio for 10 min at 22 degrees Celcius. Significant thiol and amine loss was found in beta-LQ compared to beta-LG. 4MBQ-induced modifications were identified on Cys, Lys, Arg, His and Trp in beta-LQ by LC-MS/MS after tryptic hydrolysis. The concentration of Cys-4MC adduct was determined after total acidic hydrolysis of beta-LQ. No significant differences between beta-LG and beta-LQ on in vitro digestibility were observed by assessment with SDS-PAGE, degree of hydrolysis and LC-MS/MS peptide intensities. Nine of the in vitro digested peptides from beta-LG and beta-LQ were identified as bioactive from a database search. Cys-4MC adduct was found to be bioaccessible after in vitro digestion.
HostingRepository	MassIVE
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-02-10_01:52:20.469.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kasper Engholm-Keller
SpeciesList	scientific name: Bos taurus; common name: cattle; NCBI TaxID: 9913;
ModificationList	unknown modification
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-19 05:59:27	ID requested
⏵ 1	2022-02-10 01:52:20	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: beta-lactoglobulin, Digestibility, protein-polyphenol covalent adducts, 4-methylcatechol, 4-methylbenzoquinone

submitter keyword: beta-lactoglobulin, Digestibility, protein-polyphenol covalent adducts, 4-methylcatechol, 4-methylbenzoquinone

Contact List

Marianne Nissen Lund
contact affiliation	Department of Food Science, University of Copenhagen
contact email	mnl@food.ku.dk
lab head
Kasper Engholm-Keller
contact affiliation	University of Copenhagen
contact email	kasperengholmkeller@gmail.com
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088002/