PXD027856 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Calcineurin increases expression of aldosterone synthase via dephosphorylation of NFATc4 |
Description | The mineralocorticoid hormone, aldosterone, is secreted by the adrenal zona glomerulosa (ZG) in response to high plasma K+ and hypovolemia and promotes renal Na+ reabsorption and K+ secretion. Hence, the regulation of aldosterone secretion is critical for the control of ion homeostasis and blood pressure. While the kinase pathways regulating aldosterone production are well studied, little is known about the involved phosphatases. Using the human adrenocortical carcinoma cell line NCI-H295R, we found that the mRNA expression of the aldosterone synthase increases significantly within 6 hours after K+ exposure. This increase was inhibited in a dose-dependent manner by the calcineurin inhibitors tacrolimus and cyclosporine A. Calcineurin (Cn) is a serine-threonine-specific, Ca2+ and CaM-activated protein phosphatase essential for lymphocyte, neuronal and cardiac function. The physiologic role of Cn in the ZG cells and the molecular pathways by which Cn regulates the K+-stimulated secretion of aldosterone are unknown. To answer these questions, we stimulated NCI-H295R cells with K+ with or without Tacrolimus and studied the phosphorylation pattern of cytoplasmic proteins by phospho-proteomics. We generated a map of the changes in the Ser/Thr phosphorylation in adrenocortical cells upon stimulation with K+ and identified Cn-regulated phosphoproteins. |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-25 |
AnnouncementXML | Submission_2023-05-24_21:03:57.837.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MesutBerber |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-11 04:27:15 | ID requested | |
⏵ 1 | 2023-05-24 21:03:58 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: NCI-H295R, calcineruin, tacrolimus, aldosterone, Cn,Potassium, adrenal gland |
Contact List
DavidPenton |
contact affiliation | University of Zurich, Switzerland |
contact email | david.pentonribas@uzh.ch |
lab head | |
MesutBerber |
contact affiliation | Institute of Anatomy, University of Zurich |
contact email | mesut.berber@anatomy.uzh.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027856
- Label: PRIDE project
- Name: Calcineurin increases expression of aldosterone synthase via dephosphorylation of NFATc4