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PXD027856

PXD027856 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCalcineurin increases expression of aldosterone synthase via dephosphorylation of NFATc4
DescriptionThe mineralocorticoid hormone, aldosterone, is secreted by the adrenal zona glomerulosa (ZG) in response to high plasma K+ and hypovolemia and promotes renal Na+ reabsorption and K+ secretion. Hence, the regulation of aldosterone secretion is critical for the control of ion homeostasis and blood pressure. While the kinase pathways regulating aldosterone production are well studied, little is known about the involved phosphatases. Using the human adrenocortical carcinoma cell line NCI-H295R, we found that the mRNA expression of the aldosterone synthase increases significantly within 6 hours after K+ exposure. This increase was inhibited in a dose-dependent manner by the calcineurin inhibitors tacrolimus and cyclosporine A. Calcineurin (Cn) is a serine-threonine-specific, Ca2+ and CaM-activated protein phosphatase essential for lymphocyte, neuronal and cardiac function. The physiologic role of Cn in the ZG cells and the molecular pathways by which Cn regulates the K+-stimulated secretion of aldosterone are unknown. To answer these questions, we stimulated NCI-H295R cells with K+ with or without Tacrolimus and studied the phosphorylation pattern of cytoplasmic proteins by phospho-proteomics. We generated a map of the changes in the Ser/Thr phosphorylation in adrenocortical cells upon stimulation with K+ and identified Cn-regulated phosphoproteins.
HostingRepositoryPRIDE
AnnounceDate2023-05-25
AnnouncementXMLSubmission_2023-05-24_21:03:57.837.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMesutBerber
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-11 04:27:15ID requested
12023-05-24 21:03:58announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
submitter keyword: NCI-H295R, calcineruin, tacrolimus, aldosterone, Cn,Potassium, adrenal gland
Contact List
DavidPenton
contact affiliationUniversity of Zurich, Switzerland
contact emaildavid.pentonribas@uzh.ch
lab head
MesutBerber
contact affiliationInstitute of Anatomy, University of Zurich
contact emailmesut.berber@anatomy.uzh.ch
dataset submitter
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