PXD027835

PXD027835 is an original dataset announced via ProteomeXchange.

Title	Growth Rate Dependent and Nutrient-specific Gene Expression Resource Allocation in Fission Yeast
Description	Cellular resources are limited and their relative allocation to gene expression programmes determines physiological states and global properties such as the growth rate. Quantitative studies using various growth conditions have singled out growth rate as a major physiological variable explaining relative protein abundances. Here, we used the simple eukaryote Schizosaccharomyces pombe to determine the importance of growth rate in explaining relative changes in protein and mRNA levels during growth on a series of non-limiting nitrogen sources. Although half of fission yeast genes were significantly correlated with the growth rate, this came alongside widespread nutrient-specific regulation. Proteome and transcriptome often showed coordinated regulation but with notable exceptions, such as metabolic enzymes. Genes positively correlated with growth rate participated in every level of protein production with the notable exception of RNA polymerase II, whereas those negatively correlated mainly belonged to the environmental stress response programme. Critically, metabolic enzymes, which represent ~55-70% of the proteome by mass, showed mainly condition-specific regulation. Specifically, many enzymes involved in glycolysis and NAD-dependent metabolism as well as the fermentative and respiratory pathways were condition-dependent and not consistently correlated with growth. In summary, we provide a rich account of resource allocation to gene expression in a simple eukaryote, advancing our basic understanding of the interplay between growth-rate dependent and nutrient-specific gene expression.
HostingRepository	PRIDE
AnnounceDate	2022-02-24
AnnouncementXML	Submission_2022-02-24_06:34:17.436.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Holger Kramer
SpeciesList	scientific name: Schizosaccharomyces pombe OY26; NCBI TaxID: 649908;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-09 23:47:31	ID requested
⏵ 1	2022-02-24 06:34:17	announced

Dataset with its publication pending

submitter keyword: Fission yeast, Schizosaccharomyces pombe, Resource allocation, Proteomics, Transcriptomics, Gene expression

Holger Kramer
contact affiliation	MRC London Institute of Medical Sciences Proteomics & Metabolomics Facility Hammersmith Hospital Campus Du Cane Road London, W12 0NN United Kingdom
contact email	h.kramer@lms.mrc.ac.uk
lab head
Holger Kramer
contact affiliation	MRC London Institute of Medical Sciences
contact email	h.kramer@lms.mrc.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027835
     1. Label: PRIDE project
     2. Name: Growth Rate Dependent and Nutrient-specific Gene Expression Resource Allocation in Fission Yeast