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PXD027656

PXD027656 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntratumoral magnetic hyperthermia in combination with 5-fluorouracil induces thrombogenic collagen fibers, cellular stress, and immune responses in heterotopic human colon cancer in mice
DescriptionIn this study we looked for the main protein pathway regulators which were responsible for the therapeutic impact on colon cancers when combining magnetic hyperthermia with the chemo-therapeutic agent 5-fluorouracil (5FU). In this context, chitosan-coated magnetic nanoparticles (MNP) functionalized with 5FU were intratumorally injected into subcutaneous human colon cancer xenografts (HT-29) in mice and exposed to an alternating magnetic field. A decreased tu-mor growth was found particularly for the combined thermo-chemotherapy vs. the correspond-ing monotherapies. By using computational analysis of the tumor proteome, we found upregu-lated functional pathway categories termed “cellular stress and injury”, “intracellular second messenger and nuclear receptor signaling”, “immune responses”, and “growth proliferation and development”. We predict TGF-beta, and other mediators, as important upstream regulators. In conclusion our findings show that the combined thermo-chemotherapy induces thrombogenic collagen fibers which are able to impair tumor nutrient supply. Further on, we associate several responses to the recognition of damage associated molecular patterns (DAMPs) by phagocytic cells, which immigrate into the tumor area. The activation of some pathways associated with cell survival implies the necessity to conduct multiple therapy sessions in connection with a corre-sponding monitoring, which could possibly be conducted on the base of the identified protein regulators.
HostingRepositoryPRIDE
AnnounceDate2021-11-10
AnnouncementXMLSubmission_2021-11-10_05:01:52.022.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEmilio Cirri
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-30 00:18:19ID requested
12021-11-10 05:01:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: tumor, magnetic hyperthermia, 5FU, proteomics
Contact List
Ingrid Hilger
contact affiliationInstitute of Diagnostic and Interventional Radiology, Jena University Hospital – Friedrich Schiller Uni-versity Jena, Am Klinikum 1, D-07740 Jena, Germany
contact emailingrid.hilger@med.uni-jena.de
lab head
Emilio Cirri
contact affiliationLeibniz Institute on Ageing Fritz Lipmann Institute Jena
contact emailemilio.cirri@fli-leibniz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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