PXD027656 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Intratumoral magnetic hyperthermia in combination with 5-fluorouracil induces thrombogenic collagen fibers, cellular stress, and immune responses in heterotopic human colon cancer in mice |
Description | In this study we looked for the main protein pathway regulators which were responsible for the therapeutic impact on colon cancers when combining magnetic hyperthermia with the chemo-therapeutic agent 5-fluorouracil (5FU). In this context, chitosan-coated magnetic nanoparticles (MNP) functionalized with 5FU were intratumorally injected into subcutaneous human colon cancer xenografts (HT-29) in mice and exposed to an alternating magnetic field. A decreased tu-mor growth was found particularly for the combined thermo-chemotherapy vs. the correspond-ing monotherapies. By using computational analysis of the tumor proteome, we found upregu-lated functional pathway categories termed “cellular stress and injury”, “intracellular second messenger and nuclear receptor signaling”, “immune responses”, and “growth proliferation and development”. We predict TGF-beta, and other mediators, as important upstream regulators. In conclusion our findings show that the combined thermo-chemotherapy induces thrombogenic collagen fibers which are able to impair tumor nutrient supply. Further on, we associate several responses to the recognition of damage associated molecular patterns (DAMPs) by phagocytic cells, which immigrate into the tumor area. The activation of some pathways associated with cell survival implies the necessity to conduct multiple therapy sessions in connection with a corre-sponding monitoring, which could possibly be conducted on the base of the identified protein regulators. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-10 |
AnnouncementXML | Submission_2021-11-10_05:01:52.022.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Emilio Cirri |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-30 00:18:19 | ID requested | |
⏵ 1 | 2021-11-10 05:01:52 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: tumor, magnetic hyperthermia, 5FU, proteomics |
Contact List
Ingrid Hilger |
contact affiliation | Institute of Diagnostic and Interventional Radiology, Jena University Hospital – Friedrich Schiller Uni-versity Jena, Am Klinikum 1, D-07740 Jena, Germany |
contact email | ingrid.hilger@med.uni-jena.de |
lab head | |
Emilio Cirri |
contact affiliation | Leibniz Institute on Ageing Fritz Lipmann Institute Jena |
contact email | emilio.cirri@fli-leibniz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027656
- Label: PRIDE project
- Name: Intratumoral magnetic hyperthermia in combination with 5-fluorouracil induces thrombogenic collagen fibers, cellular stress, and immune responses in heterotopic human colon cancer in mice