PXD027586 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-dependent Mitophagy |
Description | Mitophagy, the selective degradation of mitochondria by autophagy, affects defective mitochondria following damage or stress. At the onset of mitophagy, parkin ubiquitylates proteins on mitochondrial outer membrane (MOM). While the role of parkin at the onset of mitophagy is well understood, less is known about its activity during later stages of the process. Here we used HeLa cells expressing catalytically active or inactive parkin to perform temporal analysis of the proteome, ubiquitylome and phosphoproteome during 18 hours after induction of mitophagy by mitochondrial uncoupler CCCP. Abundance profiles of proteins downregulated in parkin-dependent manner revealed a stepwise, “outside-in” directed degradation of mitochondrial subcompartments. While ubiquitylation of MOM proteins was enriched among early parkin-dependent targets, numerous mitochondrial inner membrane, matrix and cytosolic proteins were also found ubiquitinated at later stages of mitophagy. Phosphoproteome analysis revealed a possible cross-talk between phosphorylation and ubiquitylation during mitophagy on several key parkin targets, such as VDAC1/2. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:52:55.342.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nicolas Nalpas |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine; phosphorylated residue |
Instrument | Q Exactive HF; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-27 07:26:32 | ID requested | |
1 | 2022-01-17 02:33:17 | announced | |
⏵ 2 | 2023-11-14 08:52:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Quantitative proteomics, Mitochondria, Mitophagy, Ubiquitin,Parkin |
Contact List
Prof. Dr. Boris Macek |
contact affiliation | Chair, Quantitative Proteomics Director, Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany |
contact email | boris.macek@uni-tuebingen.de |
lab head | |
Nicolas Nalpas |
contact affiliation | Tuebingen University |
contact email | nalpas.nicolas@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027586
- Label: PRIDE project
- Name: Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-dependent Mitophagy