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PXD027586

PXD027586 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTemporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-dependent Mitophagy
DescriptionMitophagy, the selective degradation of mitochondria by autophagy, affects defective mitochondria following damage or stress. At the onset of mitophagy, parkin ubiquitylates proteins on mitochondrial outer membrane (MOM). While the role of parkin at the onset of mitophagy is well understood, less is known about its activity during later stages of the process. Here we used HeLa cells expressing catalytically active or inactive parkin to perform temporal analysis of the proteome, ubiquitylome and phosphoproteome during 18 hours after induction of mitophagy by mitochondrial uncoupler CCCP. Abundance profiles of proteins downregulated in parkin-dependent manner revealed a stepwise, “outside-in” directed degradation of mitochondrial subcompartments. While ubiquitylation of MOM proteins was enriched among early parkin-dependent targets, numerous mitochondrial inner membrane, matrix and cytosolic proteins were also found ubiquitinated at later stages of mitophagy. Phosphoproteome analysis revealed a possible cross-talk between phosphorylation and ubiquitylation during mitophagy on several key parkin targets, such as VDAC1/2.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:52:55.342.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNicolas Nalpas
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine; phosphorylated residue
InstrumentQ Exactive HF; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-27 07:26:32ID requested
12022-01-17 02:33:17announced
22023-11-14 08:52:55announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Quantitative proteomics, Mitochondria, Mitophagy, Ubiquitin,Parkin
Contact List
Prof. Dr. Boris Macek
contact affiliationChair, Quantitative Proteomics Director, Proteome Center Tuebingen Interfaculty Institute for Cell Biology University of Tuebingen Auf der Morgenstelle 15 72076 Tuebingen Germany
contact emailboris.macek@uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliationTuebingen University
contact emailnalpas.nicolas@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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